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Production and potential applications of a xylanase from a new strain of Streptomyces cuspidosporus
, M. Uma Maheswari
Published in Springer Nature Switzerland AG
2000
Volume: 16
   
Issue: 3
Pages: 257 - 263
Abstract
Enzyme production by a new mesophilic Streptomyces isolate was investigated which grew optimally on 1% (w/v) xylan and 10% (w/v) wheat bran at pH 7 and 37 °C. Xylan induced only CMCase (0.29 U/ml) besides xylanase (22-35 U/ml, 40-49 U/mg protein). Wheat bran induced xylanase (105 U/ml, 17.5 U/mg protein), CMCase (0.74 U/ml), β-xylosidase (0.009 U/ml), β-glucosidase (0.026 U/ml), α-L-arabinofuranosidase (0.049 U/ml), amylase (1.6 U/ml) and phytase (0.432 U/ml). The isolate was amenable to solid state cultivation and produced increased levels of xylanase (146 U/ml, 28 U/mg protein). The pH and temperature optima of the crude xylanase activity were 5.5 and 65 °C respectively. The pI was 6.0 as determined by PEG precipitation. The crude enzyme was applied in treatment of paper pulp and predigestion of poultry feed and was found to be effective in releasing sugars from both and soluble phosphorus from the latter.
About the journal
JournalData powered by TypesetWorld Journal of Microbiology and Biotechnology
PublisherData powered by TypesetSpringer Nature Switzerland AG
ISSN09593993
Open AccessNo
Concepts (17)
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    ALPHA ARABINOFURANOSIDASE
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    Amylase
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    BETA GLUCOSIDASE
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    BRAN
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    Culture medium
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    Enzyme activity
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    Enzyme synthesis
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    ENZYMIC TREATMENT
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    GENETIC STRAIN
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    pH
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    PHYTASE
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    Temperature
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    Wheat
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    XYLAN 1,4 BETA XYLOSIDASE
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    XYLAN ENDO 1,3 BETA XYLOSIDASE
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    Xylan
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    STREPTOMYCES CUSPIDOSPORUS