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Plasmonic biosensors for bacterial endotoxin detection on biomimetic C-18 supported fiber optic probes
Hariharan Manoharan,
Published in Elsevier Ltd
2019
PMID: 30684858
Volume: 129
   
Pages: 79 - 86
Abstract
Bacterial endotoxins such as lipopolysaccharides (LPS) are major contaminants of most pharmaceutical and consumer products. We report an antibiotic-mediated plasmonic biosensor for LPS detection based on a facile U-bent fiber optic probe (UFOP) technology. Biomimetic self-assembled layer of octadecyltrichlorosilanes (OTS) were functionalized on the surface of optical fiber probes to hydrophobically entrap LPS from aqueous solutions. The binding of LPS molecules was monitored in real-time by measuring the change in refractive index (RI) in the evanescent layer. To add specificity and signal amplification, the bound LPS molecules were further tagged with antimicrobial polymyxin-B conjugated gold nanoparticles (PMB-AuNPs) in a sandwich format. The assay was extensively optimized by investigating the role of experimental parameters like OTS concentration, incubation time and addition of a silver reduction step at the end of the assay. The lower limit of detection (LOD) for LPS was found to be 0.4 ng/mL with a 36-fold improved sensitivity upon silver enhancement. The total assay time was 1 h. The assay was also found to be highly specific in the presence of common biopharmaceutical components and could thus serve as an efficient endotoxin detection platform for quality control testing during therapeutic development. © 2019 Elsevier B.V.
About the journal
JournalData powered by TypesetBiosensors and Bioelectronics
PublisherData powered by TypesetElsevier Ltd
ISSN09565663
Open AccessNo
Concepts (55)
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    Biomimetics
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    Biosensors
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    Fiber optic sensors
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    Gold nanoparticles
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    Metal nanoparticles
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    Molecules
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    Optical fibers
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    Plasmonics
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    Plasmons
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    Probes
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    Quality control
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    Refractive index
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    Silver
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    BENT FIBER
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    Endotoxin
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    Lipopolysaccharides
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    POLYMYXIN B
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    SANDWICH ASSAYS
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    Fiber optics
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    Gold nanoparticle
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    Lipopolysaccharide
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    OCTADECYLTRICHLOROSILANE
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    POLYMYXIN B
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    Silane derivative
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    Unclassified drug
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    Biomimetic material
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    GLASS FIBER
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    Gold
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    Metal nanoparticle
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    OCTADECYLTRICHLOROSILANE
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    Water
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    Article
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    Concentration (parameter)
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    ENDOTOXIN DETECTION
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    Hydrophobicity
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    Limit of detection
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    Plasmonic biosensor
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    Process monitoring
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    Process optimization
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    Refraction index
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    Sensitivity analysis
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    U BENT FIBER OPTIC PROBE
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    Chemical phenomena
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    Chemistry
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    Devices
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    Equipment design
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    Genetic procedures
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    REFRACTOMETRY
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    Biomimetic materials
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    Biosensing techniques
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    FIBER OPTIC TECHNOLOGY
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    Hydrophobic and hydrophilic interactions
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    POLYMYXIN B
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    REFRACTOMETRY
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    Silanes