A suitable simple model tested by experiments is required to address complex biological reaction like esterase synthesis by Saccharomyces cerevisiae. Such an approach might be the answer to a proper bioprocessing strategy. In this regard, a logistic model for esterase production from Saccharomyces cerevisiae has been developed, which predicts well the cell mass, the carbon source (glucose) consumption, and the esterase activity. The accuracy of the model has been statistically examined by using the Student's t-test. The parameter sensitivity analysis showed that all five parameters (μm, Ks, Xm, Yx/s, and Yp/x) have significant influence on the predicted values of esterase activity. © The Korean Society for Microbiology and Biotechnology.