Hydroxyapatite has been synthesized using eggshell membrane as bio-template along with the assistance of microwave heating. Eggshell membrane is infiltrated with calcium and phosphorous precursors before heating it in a domestic microwave oven. Hydroxyapatite formed retained the interwoven hierarchical structure of the eggshell membrane. Structural analysis was performed using X-ray diffraction, and the microstructural features are characterized by scanning electron microscopy. Distinct morphological features were present in microwave processed hydroxyapatite when compared to the conventionally processed one. Transmission electron microscopic investigations and BET surface area analysis were also carried out. © 2018 Elsevier Ltd and Techna Group S.r.l.