Commercially pure titanium was ball milled (BM) in a planetary mill for various time intervals to obtain nanocrystalline particles. The milled samples were characterized using X-ray diffractometry (XRD), scanning electron microscopy (SEM), energy dispersive analysis of X-rays (EDAX), transmission electron microscopy (TEM) and porosimetry. The samples were also compacted and sintered for in vitro bioactivity assessment in simulated body fluid (SBF) for up to 4 weeks. The hydroxyapatite formed was characterized using SEM and XRD techniques. It was observed that apatite formed on the porous structure was dense and homogenous when compared to that formed on bulk titanium. The morphology of the apatite was globular and the Ca/P ratio from EDAX was found to be 1.66 which is close to that of apatite phase. Human osteoblast cells adhered on sintered pellets for 2 and 4 days showed enhanced attachment and spreading compared to bulk titanium.