In this paper, twisted shift-invariant spaces in L2(ℝ2n) are introduced. Characterizations of orthonormal system and Bessel sequence of twisted translates in L2(ℝ2n) are obtained in terms of Weyl transform. These results appear to be totally different from the classical case of characterizations of orthonormal systems and Bessel sequences of translates in L2(ℝn). Further, characterizations of frames, Riesz basis in twisted shift-invariant spaces in L2(ℝ2n)are also obtained. © 2015 Elsevier Inc.