Desorption electrospray ionization was employed for fast and direct ambient detection of the anti-tumor drug, camptothecin, and its derivative, 9-methoxycamptothecin in Nothapodytes nimmoniana. Different parts of the plant such as leaves, stems and bark were examined. The ion intensities suggest that the concentration in bark is higher than that in the leaves and stems. The method does not require any sample preparation or preseparation. The identity of the alkaloids was further confirmed by tandem mass spectrometry. © The Royal Society of Chemistry 2011.

Pradeep Thalappil

Department Of Chemistry

Amitava Srimany

Hemanta R. Naik

9 METHOXYCAMPTOTHECIN

9-METHOXYCAMPTOTHECIN

antineoplastic agent

CAMPTOTHECIN

drug derivative

angiosperm

article

BARK

chemistry

Economics

electrospray mass spectrometry

methodology

plant leaf

plant stem

Angiosperms

Antineoplastic Agents, Phytogenic

PLANT BARK

PLANT LEAVES

PLANT STEMS

Spectrometry, Mass, Electrospray Ionization

IIT Madras is a public technical and research university located in Chennai, Tamil Nadu. Founded in 1959, it is recognised as an Institute of National Importance.

IIT Madras has been ranked as the top engineering institute in India for four years in a row by the National Institutional Ranking Framework of the MHRD

It currently offers undergraduate, postgraduate and research degrees across 16 disciplines in Engineering, Sciences, Humanities and Management. About 596 faculty belonging to science and engineering departments and centres of the Institute are engaged in teaching, research and industrial consultancy.

IIT Madras

Analyst

Areca nut (seed of Areca catechu) is consumed by people from different parts of Asia, including India. The four major alkaloids present in areca nut are arecoline, arecaidine, guvacoline and guvacine. Upon cutting, the nut reveals two kinds of regions; white and brown. In our present study, we have monitored the formation of these two regions within the nut during maturation, using the non-invasive techniques of magnetic resonance imaging (MRI) and volume localized magnetic resonance spectroscopy (MRS). Electrospray ionization mass spectrometry (ESI MS) and desorption electrospray ionization mass spectrometry (DESI MS) imaging have been used to study the associated change in the alkaloid contents of these two regions during the growth of the nut. Our study reveals that white and brown regions start forming within the nut when the liquid within starts solidifying. At the final stage of maturity, arecoline, arecaidine and guvacoline get segregated in the brown region whereas guvacine gets to the white region of the nut. The transport of molecules with maturity and corresponding pattern formation are expected to be associated with a multitude of physiochemical changes. © 2016 Elsevier Ltd. All rights reserved.

Phytochemistry

Developmental patterning and segregation of alkaloids in areca nut (seed of Areca catechu) revealed by magnetic resonance and mass spectrometry imaging

DESI MS, has been successfully employed for the analysis of molecules from a wide variety of surfaces without prior sample treatment. The efficiency of DESI MS relies on various parameters. However, those that critically affect the ionization of biological samples include: the solvent system and the sample or sample spotting surfaces. These parameters gain unequivocal dominance specially whilst dealing with sensitive and intricate biological samples. This review is meant to capture the attention of the DESI-MS researchers towards the crucial role of the solvent and sample spotting surfaces for successful biological DESI-MS. This review highlights these parameters as the backbone of the breakthroughs achieved in the analysis of biological materials of plant, bacterial, animal and human origins. © 2016 Elsevier B.V..

TrAC - Trends in Analytical Chemistry

Biological Desorption Electrospray Ionization Mass Spectrometry (DESI MS) - unequivocal role of crucial ionization factors, solvent system and substrates

Rationale Various disease conditions, particularly tumours, can be understood easily by studying changes in the lipid profile of cells. While lipid profiles of tissues have been recorded by desorption electrospray ionization mass spectrometric (DESI-MS) imaging, there is paucity in standardized protocols for sample preparation involving cell cultures to generate reliable results. In this study, we report a method for the direct analysis of lipids from cultured cells by incorporating them onto Whatman 42 filter paper as a substrate for reliable DESI-MS analysis. Methods The WERI-RB1 cell line was spotted on commonly used substrates for DESI-MS analysis, such as glass slides, Teflon coated glass slides, thin layer chromatography (TLC) plates, and Whatman 42 filter paper. A comparison of mass spectrometric images with two different lipids was made to understand the behaviour of different surfaces when the same sample was spotted on them. Relative intensities of different lipid peaks in the WERI-RB1 cell line were compared and relative lipid abundances were also compared across two different human retinoblastoma cell lines; WERI-RB1 and Y79. Results The study demonstrates that good lipid signals can be obtained by DESI-MS when the cells are spotted on Whatman 42 filter paper. Tandem mass spectrometry was performed to identify the lipids as glycerophosphocholines (PC). Better lipid images from assembly of cells were obtained with distinct boundary when they were spotted on Whatman 42 filter paper than other surfaces. Conclusions We demonstrate the use of a simple substrate for reliable DESI-MS analysis of cultured cells. This method has the potential to understand various interactions of cells with other external agents. The current method would help in the application of DESI-MS for biology in general and medical sciences in particular. Copyright © 2015 John Wiley & Sons, Ltd.

Rapid Communications in Mass Spectrometry

Identification of effective substrates for the direct analysis of lipids from cell lines using desorption electrospray ionization mass spectrometry

Tulsi or Holy Basil (Ocimum sanctum Linn) is a medicinally important plant. Ursolic acid (UA) and oleanolic acid (OA) are among its major constituents which account for many medicinal activities of the plant. In the present work, we deployed a new ambient ionization method, leaf spray ionization, for rapid detection of UA, OA and their oxidation products from tulsi leaves. Tandem electrospray ionization mass spectrometry (ESI-MS) has been performed on tulsi leaf extracts in methanol to establish the identity of the compounds. We probed changes occurring in the relative amounts of the parent compounds (UA and OA) with their oxidized products and the latter show an increasing trend upon ageing. The findings are verified by ESI-MS analysis of tulsi leaf extracts, which shows the same trend proving the reliability of the leaf spray method. © 2012 The Royal Society of Chemistry.

Rapid identification of molecular changes in tulsi (Ocimum sanctum Linn) upon ageing using leaf spray ionization mass spectrometry

Analytical Letters

Determination of Camptothecin and 10-Hydroxycamptothecin inCamptotheca acuminataby LC-ESI-MS/MS

Chemical Reviews

Mass Spectrometric Imaging for Biomedical Tissue Analysis

Journal of Pharmaceutical and Biomedical Analysis

Metabolite fingerprinting of Camptotheca acuminata and the HPLC–ESI-MS/MS analysis of camptothecin and related alkaloids

The Analyst

Desorption electrospray ionization and other ambient ionization methods: current progress and preview

Analytical Chemistry

Handheld Miniature Ion Trap Mass Spectrometers

Direct analysis of camptothecin from Nothapodytes nimmoniana by desorption electrospray ionization mass spectrometry (DESI-MS)

Journal	Data powered by TypesetAnalyst
Publisher	Data powered by TypesetRoyal Society of Chemistry
ISSN	00032654
Open Access	No