A mixed bacterial culture consisted of Staphylococcus sp., Bacillus circulans-I and -II has been enriched from contaminated soil collected from the vicinity of an endosulfan processing industry. The degradation of endosulfan by mixed bacterial culture was studied in aerobic and facultative anaerobic conditions via batch experiments with an initial endosulfan concentration of 50 mg/L. After 3 weeks of incubation, mixed bacterial culture was able to degrade 71.58 ± 0.2% and 75.88 ± 0.2% of endosulfan in aerobic and facultative anaerobic conditions, respectively. The addition of external carbon (dextrose) increased the endosulfan degradation in both the conditions. The optimal dextrose concentration and inoculum size was estimated as 1 g/L and 75 mg/L, respectively. The pH of the system has significant effect on endosulfan degradation. The degradation of alpha endosulfan was more compared to beta endosulfan in all the experiments. Endosulfan biodegradation in soil was evaluated by miniature and bench scale soil reactors. The soils used for the biodegradation experiments were identified as clayey soil (CL, lean clay with sand), red soil (GM, silty gravel with sand), sandy soil (SM, silty sand with gravel) and composted soil (PT, peat) as per ASTM (American society for testing and materials) standards. Endosulfan degradation efficiency in miniature soil reactors were in the order of sandy soil followed by red soil, composted soil and clayey soil in both aerobic and anaerobic conditions. In bench scale soil reactors, endosulfan degradation was observed more in the bottom layers. After 4 weeks, maximum endosulfan degradation efficiency of 95.48 ± 0.17% was observed in red soil reactor where as in composted soil-I (moisture 38 ± 1%) and composted soil-II (moisture 45 ± 1%) it was 96.03 ± 0.23% and 94.84 ± 0.19%, respectively. The high moisture content in compost soil reactor-II increased the endosulfan concentration in the leachate. Known intermediate metabolites of endosulfan were absent in all the above degradation studies. © 2005 Elsevier B.V. All rights reserved.