Header menu link for other important links

This study demonstrates a rapid, wash-free, dip-type plasmonic fiberoptic absorbance biosensor (P-FAB) capable of zeptomole analyte detection and 6-orders of wide dynamic range. It involves a sandwich immunoassay on a compact U-bent fiber optic probe surface that works by dipping an antibody-functionalized probe into a mixture of sample and gold nanoparticle (AuNP) label suspension. The U-bent fiberoptic probes with a high evanescent wave absorbance sensitivity allow detection of the high extinction AuNP labels measured in terms of light intensity change using a pair of LED and photodetector (PD). This simple and low-cost P-FAB gives an unprecedented detection limit of 0.17 zeptomole for human immunoglobulin G (HIgG) in 25 μL buffer in just 25 min. Further silver enhancement of AuNP labels for 5 min results in a limit of quantitation (LoQ) down to 0.17 zeptomole (∼100−150 molecules in 25 μL), making P-FAB a highly sensitive and low-cost technology for pointof-care diagnostics.

About the journal
JournalData powered by TypesetSensors and Actuators B: Chemical
PublisherData powered by TypesetElsevier
Open AccessNo