Using an electron-positron collision data sample corresponding to an integrated luminosity of 2.93 fb-1 collected with the BESIII detector at a center-of-mass energy of 3.773 GeV, we search for the baryon-number and lepton-number violating decays D0→p¯e+ and D0→pe-. No obvious signals are found with the current statistics. The upper limits on the branching fractions for D0→p¯e+ and D0→pe- are set to be 1.2×10-6 and 2.2×10-6 at 90% confidence level, respectively. © 2022 authors. Published by the American Physical Society. Published by the American Physical Society under the terms of the "https://creativecommons.org/licenses/by/4.0/"Creative Commons Attribution 4.0 International license. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI. Funded by SCOAP3.