Biosorption using microbial cells as adsorbents is being seen as a cost-effective method for the removal of heavy metals from wastewaters. Biosorption studies with Phanerochaete chrysosporium were performed for copper (II), lead (II), and cadmium (II) to evaluate the effectiveness and to optimize the operational parameters using response surface methodology. The operational parameters chosen were initial metal ion concentration, pH, and biosorbent dosage. Using this method, the metal removal could be correlated to the operational parameters, and their values were optimized. The results showed fairly high adsorptive capacities for all the metals within the settings of the operational parameters. The removal efficiencies followed the order Pb > Cu > Cd. As a general trend, metal removal efficiency decreased as the initial metal ion concentration increased, and the results fitted the Langmuir and Freundlich isotherms well.

Thiyagarajan R. Swaminathan

Cadmium

Copper

Heavy metal

Lead

metal ion

Adsorption

Biosorption

concentration (parameters)

conference paper

controlled study

cost effectiveness analysis

Metal recovery

methodology

nonhuman

pH measurement

PHANEROCHAETE

waste water management

article

Biomass

bioremediation

dose response

isolation and purification

Kinetics

metabolism

Sewage

Statistics

CHRYSOSPORIUM

PHANEROCHAETE CHRYSOSPORIUM

Biodegradation, Environmental

Dose-Response Relationship, Drug

Hydrogen-Ion Concentration

Metals, Heavy

Waste Disposal, Fluid

IIT Madras is a public technical and research university located in Chennai, Tamil Nadu. Founded in 1959, it is recognised as an Institute of National Importance.

IIT Madras has been ranked as the top engineering institute in India for four years in a row by the National Institutional Ranking Framework of the MHRD

It currently offers undergraduate, postgraduate and research degrees across 16 disciplines in Engineering, Sciences, Humanities and Management. About 596 faculty belonging to science and engineering departments and centres of the Institute are engaged in teaching, research and industrial consultancy.

IIT Madras

Heavy metal removal by biosorption using Phanerochaete chrysosporium

Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

Biosorption of lead by immobilised Phanerochaete chrysosporium over two successive cycles of sorption-desorption was investigated in a continuously operated packed bed column. The breakthrough curves in each sorption cycle were measured as a function of different bed depths in the column at an inlet lead concentration of 40 mg/L. The values of various breakthrough parameters obtained in the study indicated good potential of the system in removing lead from constituent wastewaters. Kinetic and mass transfer aspects of lead biosorption in the study were also evaluated by fitting the experimental data to several breakthrough models found in the literature. And, compared to the other models, Clark model showed good predictability of the entire breakthrough data. © 2010 Inderscience Enterprises Ltd.

International Journal of Environmental Technology and Management

Biosorption of lead by the immobilised fungus Phanerochaete chrysosporium in a packed bed column

Biosorption of three divalent metals, viz., lead, copper, and cadmium in ternary aqueous mixtures was studied using Phanerochaete chrysosporium in batch shake flasks. The mixtures were prepared containing the metals at their either varying optimum or equal initial concentration combinations in aqueous solution of pH optimum to each of the metals. Following were the optimum initial concentration ranges of the metals in mixture: lead, 60-100 mg/L; copper, 20-60 mg/L; and cadmium, 5-15 mg/L. And, for varying these optimum concentration levels of the metals, a 23 full factorial design of experiments was employed. The results revealed that an increase in lead and cadmium concentrations helped in their better biosorption by the fungus, but an increase in initial copper concentration slightly diminished its removal. Statistical analysis of the results in the form of analysis of variance and Student t test gave a clear interpretation on the roles of both the individual metals and their interactions in the uptake of metals from mixture. Compared to the uptake of metals when presented individually, lead biosorption in mixture was found to be enhanced to a degree as high as 99%; on the other hand, copper and cadmium removals from mixtures were inhibited to the extent of 100% and 98%, respectively. However, this extent of inhibition or enhancement in the metal removals compared to the individual removals was less in mixtures containing all equal concentrations of the metals. © 2008 Humana Press.

Applied Biochemistry and Biotechnology

Biosorption of lead, copper, and cadmium by phanerochaete chrysosporium in ternary metal mixtures: Statistical analysis of individual and interaction effects

Adsorption of Astrazone Blue FRR (Basic Blue 69) and Teflon Blue ANL (Acid Blue 125) was investigated using a hybrid adsorbent that was prepared by pyrolysing a mixture of carbon and fly ash in 1:1 ratio. A 24 full factorial central composite design was successfully employed for experimental design and analysis of the results. The combined effect of pH, temperature, particle size and time on the dye adsorption was studied and optimized using response surface methodology. The optimum pH, temperature, particle size and time were found to be 12.8, 27.75°C, 0.0555 mm, 230 min, respectively for basic blue and those for acid blue 25 were 1.5, 27.5°C, 0.0565 mm and 245 min, respectively. Complete removal (100%) was observed for both the dyes using the hybrid adsorbent. © 2004 Elsevier B.V. All rights reserved.

Chemical Engineering Journal

Optimization of batch process parameters using response surface methodology for dye removal by a novel adsorbent

Hydrometallurgy

Biohydrometallurgical prospects

OPSEARCH

Abstracts of papers presented at the XXXII Annual Convention of Operational Research Society of India

Bioprocess Engineering

Optimisation of lignin peroxidase production from Phanerochaete chrysosporium using response surface methodology

Biotechnology Progress

Biosorption of Lead, Cadmium, and Zinc by Citrobacter Strain MCM B-181: Characterization Studies

Biohydrometallurgy and the Environment Toward the Mining of the 21st Century - Proceedings of the International Biohydrometallurgy Symposium Process Metallurgy

Present and future commercial applications of biohydrometallurgy

Journal	Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
ISSN	02732289
Open Access	No