The effect of separation of fiber orientation through the thickness of thin composites on their low velocity impact response is studied. The composites are prepared using unidirectional glass fiber reinforced epoxy through hand layup followed by vacuum bagging. The two dissimilar layups of composites considered have separation of composite layers with fibers having the same and different orientations through the thickness. The composites are subjected to low velocity impact using a drop-weight testing machine. The composites are evaluated using different performance parameters such as damage degree, first damage and maximum forces, first cracking energy, bending stiffness, elastic strain energy, elastic, residual and maximum displacements, permanent deformation, square-root delaminated area, delamination length and width, and contact duration. Among the two composites, it is observed that [90/-45/45/0] composite in which two layers with fibers having 90° and 0° orientations separating by two layers with fibers having -45° and 45° orientations, levels of deformation are lower and recorded force and square-root delaminated area are higher and lower, respectively for the same level of impact energy. Whereas, in case of [0/90/90/0] composite in which two layers with fibers having 0° orientations separating by two layers with fibers having 90° orientations, recorded force is lower and deformation and square-root delaminated area are higher. The [0/90/90/0] composite is having a comparatively more lateral spread of delamination and inter-layer opening than that of [90/-45/45/0] composite considering extensional and bending stiffnesses along longitudinal (A11, D11) and transverse (A22, D22) directions. This facilitates that lateral spread of damage within composite can be decreased by separating two layers of composite with 90° and 0° fiber orientations by two layers with -45° and 45° fiber orientations, i.e., [90/-45/45/0] composite. © 2019Materials science; Mechanical engineering; Composites; Low velocity impact; Delamination; Energy absorbed; Structural analysis; Composite materials; Materials characterization; Mechanical property; Structural behavior © 2019