The synthesis of pectinase is investigated using six species of Aspergillus, with five media differing either in their carbon sources or level of carbon source(s). Five of the six species used, synthesized appreciable amounts of pectinase in the media containing sugars. Pectinase synthesis was highest for A. niger, NCIM 548, with all the sugar containing media. A. foetidus, NCIM 510, was the only one among the organisms studied, that responded well to the medium containing pectin in the absence of additional sugars supplied in the medium. © 1995 Springer-Verlag.

T Panda

Department Of Chemical Engineering

Sushma R. Nair

carbohydrate

POLYGALACTURONASE

article

Aspergillus

Aspergillus niger

controlled study

culture medium

enzyme synthesis

fungus growth

nonhuman

priority journal

species difference

IIT Madras is a public technical and research university located in Chennai, Tamil Nadu. Founded in 1959, it is recognised as an Institute of National Importance.

IIT Madras has been ranked as the top engineering institute in India for four years in a row by the National Institutional Ranking Framework of the MHRD

It currently offers undergraduate, postgraduate and research degrees across 16 disciplines in Engineering, Sciences, Humanities and Management. About 596 faculty belonging to science and engineering departments and centres of the Institute are engaged in teaching, research and industrial consultancy.

IIT Madras

Effect of carbon sources on the synthesis of pectinase by Aspergilli

Bioprocess Engineering

The present study is focused on the utilization of fruit and agro-industrial wastes for the production of yeast pectinases using response surface optimization techniques. Orange peel (OP), groundnut oil cake (GC), MnSO4 and incubation period (IP) were found as significant parameters for pectinase production. The central composite design of media optimization indicated that OP (5), GC (4), MnSO4 (0.08%, w/v) and IP (48 h) increased the pectinase production by 9-fold. Crude pectinase promoted the enzymatic peeling of oranges and processing of raw banana fibers. Topographical changes of enzyme treated orange and mango peels were studied using AFM which provides a new tool to unravel the role of pectin aggregation in the dissolution of middle lamella during enzymatic hydrolysis. Changes in the fingerprint regions of enzyme treated fruit substrates were observed along with the disappearance of the impurities such as waxes and pectin using FTIR spectral analysis. © 2016 Elsevier Ltd.

Biocatalysis and Agricultural Biotechnology

Enhanced production of pectinase by Saccharomyces cerevisiae isolate using fruit and agro-industrial wastes: Its application in fruit and fiber processing

Pectic transeliminases, also known as pectic lyases or pectinases, are involved in the degradation of pectic substances. They have a wide range of applications in food and textile processing. Although Aspergillus and Penicillium spp. produce pectin lyases, bacteria are the major producers of polygalacturonate lyase. The yields of pectic transeliminases are less than other pectinases. Since new applications for pectic transeliminases are emerging, an improved process for the production of these enzymes is necessary. © Springer 2005.

Biotechnology Letters

Microbial pectic transeliminases

Pectinases are a complex group of enzymes that degrade various pectic substances present in plant tissues. Pectinases have potential applications in fruit, paper and textile industries. Apart from these industrial applications, these enzymes possess biological importance in protoplast fusion technology and plant pathology. Since applications of pectinases in various fields are widening, it is important to understand the nature and properties of these enzymes for efficient and effective usage. For the past few years, vigorous research has been carried out on isolation and characterization of pectinases. New affinity matrices with improved characteristics and affinity-precipitation techniques have been developed for purification of pectinases. Recently much attention has been focused on chemical modification of pectinases and their catalytic performance by various researchers. These studies are helpful in determining key amino acid residues responsible for substrate binding, catalytic action, and physico-chemical environmental conditions for maximum hydrolysis. This short review highlights progress on purification and understanding the biochemical aspects of microbial pectinases. © 2002 Elsevier Science Ltd. All rights reserved.

Process Biochemistry

Purification and biochemical properties of microbial pectinases - A review

Pectolytic enzymes play an important role in food processing industries and alcoholic beverage industries. These enzymes degrade pectin and reduce the viscosity of the solution so that it can be handled easily. These enzymes are mainly synthesized by plants and microorganisms. Aspergillus niger is used for industrial production of pectolytic enzymes. This fungus produces polygalacturonase, polymethylgalacturonase and pectinlyase. This review mainly concerns with the production of pectolytic enzymes using different carbon sources. It also deals with the effect of operating parameters such as temperature, aeration rate, agitation and type of fermentation on the production of these enzymes.

Production of pectolytic enzymes - A review

The synthesis of pectinase using Aspergillus niger, NCIM 548, has been enhanced by optimizing the carbon and nitrogen sources present in the medium. Enzyme synthesis was about 40% more using the optimized medium than using the unoptimized medium. The study provided information on the appropriate carbon source as well as the optimum ratio of carbon to nitrogen, yielding the highest enzyme levels.

Statistical optimization of medium components for improved synthesis of pectinase by Aspergillus niger

Applied Microbiology and Biotechnology

Effects of different carbon sources on the synthesis of pectinase by Aspergillus niger in submerged and solid state fermentations

Enzyme and Microbial Technology

Strain and process for production of polygalacturonase

Variation of polygalacturonase and pectinesterase synthesis by aggregated mycelium of Aspergillus niger in dependence on the carbon source

Zentralblatt für Mikrobiologie

Synthesis of variable enzyme spectrum by immobilized mycelium of A. niger

Methods in Enzymology Biomass Part B: Lignin, Pectin, and Chitin

Assay methods for pectic enzymes

Journal	Data powered by TypesetBioprocess Engineering
Publisher	Data powered by TypesetSpringer-Verlag
ISSN	0178515X
Open Access	No