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Degradation kinetics of caffeine and related methylxanthines by induced cells of Pseudomonas sp.
Published in
2007
PMID: 17554468
Volume: 55
   
Issue: 1
Pages: 56 - 60
Abstract
In this study, the kinetics of degradation of caffeine and related methylxanthines by induced cells of Pseudomonas sp. was performed. The kinetics data showed that degradation of caffeine, theobromine, and 7-methylxanthine followed Michealis-Menten kinetics. The values of K m are low for caffeine and 7-methylxanthine and high for theobromine. Degradation of caffeine and theobromine was enhanced in the presence of NADH and NADPH, whereas the degradation of 7-methylxanthine was unaffected. Among the various metal ions tested, Fe2+ was found to enhance the rate of degradation for all three substrates, whereas Zn2+ and Cu2+ inhibited the degradation of caffeine and theobromine but not 7-methylxanthine. The differences in kinetic parameters and cofactor requirement suggest the possibility of the involvement of more than one N-demethylases in the caffeine catabolic pathway in Pseudomonas sp. The induced cells can serve as effective biocatalysts for the development of biodecaffeination techniques. © 2007 Springer Science+Business Media, LLC.
About the journal
JournalCurrent Microbiology
ISSN03438651
Open AccessNo
Concepts (32)
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    7 METHYLXANTHINE
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    Caffeine
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    Copper ion
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    Iron
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    Metal ion
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    METHYLXANTHINE DERIVATIVE
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    N DEMETHYLASE
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    Reduced nicotinamide adenine dinucleotide
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    Reduced nicotinamide adenine dinucleotide phosphate
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    THEOBROMINE
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    Zinc ion
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    Article
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    Bacterial cell
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    Biocatalyst
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    Biodegradation
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    Catabolism
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    Cell stimulation
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    Controlled study
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    Degradation kinetics
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    Michaelis constant
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    Nonhuman
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    Priority journal
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    Pseudomonas
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    Coenzymes
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    Enzyme inhibitors
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    Kinetics
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    Metals
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    NAD
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    NADP
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    THEOBROMINE
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    XANTHINES
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    Pseudomonas sp.