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Azadirachtin production by hairy root cultivation of Azadirachta indica in a modified stirred tank reactor
Published in
2012
PMID: 22580745
Volume: 35
   
Issue: 9
Pages: 1549 - 1553
Abstract
Present investigation involves hairy root cultivation of Azadirachta indica in a modified stirred tank reactor under optimized culture conditions for maximum volumetric productivity of azadirachtin. The selected hairy root line (Az-35) was induced via Agrobacterium rhizogenes LBA 920-mediated transformation of A. indica leaf explants (Coimbatore variety, India). Liquid culture of the hairy roots was developed in a modified Murashige and Skoog medium (MM2). To further enhance the productivity of azadirachtin, selected growth regulators (1.0 mg/l IAA and 0.025 mg/l GA3), permeabilizing agent (0.5 % v/v DNBP), a biotic elicitor (1 % v/v Curvularia (culture filtrate)) and an indirectly linked biosynthetic precursor (50 mg/l cholesterol) were added in the growth medium on 15th day of the hairy root cultivation period in shake flask. Highest azadirachtin production (113 mg/l) was obtained on 25th day of the growth cycle with a biomass of 21 g/l DW. Further, batch cultivation of hairy roots was carried out in a novel liquid-phase bioreactor configuration (modified stirred tank reactor with polyurethane foam as root support) to investigate the possible scale-up of the established A. indica hairy root culture. A biomass production of 15.2 g/l with azadirachtin accumulation in the hairy roots of 6.4 mg/g (97.28 mg/l) could be achieved after 25 days of the batch cultivation period, which was 27 and ̃14 % less biomass and azadirachtin concentration obtained respectively, in shake flasks. An overall volumetric productivity of 3.89 mg/(l day) of azadirachtin was obtained in the bioreactor. © Springer-Verlag 2012.
About the journal
JournalBioprocess and Biosystems Engineering
ISSN16157591
Open AccessNo
Concepts (51)
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    AGROBACTERIUM RHIZOGENES
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    Azadirachta indica
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    Azadirachtin
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    BATCH CULTIVATION
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    BIOMASS PRODUCTIONS
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    BIOSYNTHETIC PRECURSORS
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    BIOTIC ELICITORS
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    CULTIVATION PERIODS
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    Culture conditions
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    CULTURE FILTRATE
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    GROWTH CYCLE
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    GROWTH MEDIUM
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    GROWTH REGULATORS
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    HAIRY ROOT CULTURE
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    HAIRY ROOTS
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    LEAF EXPLANTS
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    Liquid culture
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    Liquid phase
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    MURASHIGE AND SKOOG MEDIUMS
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    Polyurethane foam
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    Scale-up
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    SHAKE FLASKS
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    Stirred tank reactors
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    VOLUMETRIC PRODUCTIVITY
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    Biomass
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    Bioreactors
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    Bottles
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    Ecology
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    Liquids
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    Productivity
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    Tanks (containers)
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    Cholesterol
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    DINOSEB
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    POLYURETHAN FOAM
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    Article
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    Biomass production
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    BIOTECHNOLOGICAL PRODUCTION
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    Culture medium
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    CURVULARIA
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    EXPLANT
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    HAIRY ROOT CULTURE
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    Plant leaf
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    Priority journal
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    RHIZOBIUM RHIZOGENES
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    STIRRED REACTOR
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    Azadirachta
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    Limonins
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    PLANT CELLS
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    PLANT ROOTS
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    AGROBACTERIUM RHIZOGENES
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    CURVULARIA