Header menu link for other important links
X
An alternative electron transfer process for selective detection of glucose in blood serum
Sourav Kanti Jana
Published in Elsevier B.V.
2019
Volume: 283
   
Pages: 116 - 123
Abstract
Here, we demonstrate efficient electrochemical detection of glucose in blood serum using CdS nanoribbons coated indium Tin Oxide (ITO) electrode. Main appeal of this work is to establish an alternative electron transfer (AET) process by dissolving the glucose oxidase (GOX) enzyme in the electrolyte instead of direct immobilization on the electrode's surface to demonstrate better performance of glucose sensing. In this context, we developed an experimental strategy through amperometric detection of hydrogen peroxide (H2O2) which is an intermediate reaction product of glucose oxidation by GOX present in the buffer solution. Therefore, we separately performed electrochemical detection of H2O2 by CdS nanoribbons in buffer solution followed by glucose detection with the same electrode by dissolving GOX in the buffer solution. The linear range of amperometric current measured with the same concentration of both H2O2 and glucose overlaps with each other which clearly proves that electrocatalytic reduction of both the analytes occur at the same rate on the CdS surface. Our studies reveal that dissolved GOX in buffer solution have a better performance than the GOX immobilized electrode. Furthermore, chronoamperometric detection of glucose in actual blood serum sample was performed and our results show even better performance than the commercially available glucometer. © 2018 Elsevier B.V.
About the journal
JournalData powered by TypesetSensors and Actuators, B: Chemical
PublisherData powered by TypesetElsevier B.V.
ISSN09254005
Open AccessNo
Concepts (29)
  •  related image
    Blood
  •  related image
    Cadmium sulfide
  •  related image
    Chemical detection
  •  related image
    Chronoamperometry
  •  related image
    Dissolution
  •  related image
    Electrochemical electrodes
  •  related image
    Electrolytes
  •  related image
    Electron transitions
  •  related image
    Electron transport properties
  •  related image
    ENZYME ELECTRODES
  •  related image
    Enzyme immobilization
  •  related image
    Glucose
  •  related image
    Glucose sensors
  •  related image
    Hydrogen peroxide
  •  related image
    Ii-vi semiconductors
  •  related image
    Nanoribbons
  •  related image
    Oxidation
  •  related image
    Peroxides
  •  related image
    Reaction intermediates
  •  related image
    TIN OXIDES
  •  related image
    CDS NANORIBBON
  •  related image
    ELECTROCATALYTIC REDUCTION
  •  related image
    Electrochemical biosensor
  •  related image
    Electrochemical detection
  •  related image
    Electron transfer
  •  related image
    Electron transfer process
  •  related image
    INDIUM TIN OXIDE ELECTRODES
  •  related image
    INTERMEDIATE REACTIONS
  •  related image
    Glucose oxidase