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A theory on the origin of cooperativity in DNA renaturation kinetics
Published in Elsevier
2003
PMID: 14556905
Volume: 106
   
Issue: 2
Pages: 173 - 178
Abstract

Theoretical proof for the existence of cooperativity (experimental evidences have already been reported in our earlier work: Biochem. Biophys. Res. Commun., 293 (2002) 870) in the phenomenon of DNA renaturation has been obtained using a generalized birth-death master equation approach. Results have shown that no special searching mechanism (i.e. a facilitated mechanism) was necessary for complementary DNA strands to find their correct-contacts but searching by a 'random jump' method was enough to explain not only the observed cooperativity phenomenon but also the magnitude of correct-contact-forming rate constant. Impact of cooperativity phenomenon on length of primers and their specificity in polymerase chain reaction has also been discussed.

About the journal
JournalData powered by TypesetBiophysical Chemistry
PublisherData powered by TypesetElsevier
ISSN0301-4622
Open AccessNo