The microenvironmental dependence of excited state prototropism of 1-naphthol and the corresponding changes in its fluorescence emission is utilized to monitor the acyl chain melting phase transition behavior of liposome membrane made from human erythrocyte lipids. A sharp increase in the ratio of neutral/anionic form fluorescence intensity is noticed at the phase transition temperature (19 °C). This provides a convenient method for obtaining phase transition temperature in lipid membranes. The membrane modifying effect of cholesterol on the erythrocyte liposome is successfully sensed by 1-naphthol fluorescence.

Ashok Kumar Mishra

Department Of Chemistry

Biological membranes

Electron energy levels

Emission spectroscopy

Fluorescence

Lipids

Phase Transitions

Protons

erythrocyte

Naphthol

Phospholipids

Proton transfer

Cholesterol

1 naphthol

1-NAPHTHOL

fluorescent dye

liposome

naphthol derivative

article

chemistry

erythrocyte membrane

Human

metabolism

spectrofluorometry

Temperature

Fluorescent Dyes

Humans

Liposomes

Naphthols

Spectrometry, Fluorescence

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IIT Madras

1-Naphthol as an excited state proton transfer fluorescent probe for erythrocyte membranes.

Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

This work uses the dynamics of the excited state proton transfer of 1-naphthol to water as a basis for understanding the temperature-dependent changes in the water accessibility of the niosome membrane domain. The decrease in the neutral form fluorescence intensity of membrane bound 1-naphthol, the significant drop in the membrane-bound anion form fluorescence lifetime, and the changes in the fluorescence lifetime distribution plots of the two prototropic forms of 1-naphthol indicate a regular increase in the water penetration in the interfacial region of the niosome membrane domain, whereas the dry core regions appear unaffected with increasing temperature (10-60 °C). Unlike some liposomes and niosomes, these niosomes do not show thermotropic phase transition behavior. © 2017 The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.

Fulltext

New Journal of Chemistry

Temperature-dependent water penetration in Tween20:cholesterol niosome membrane: A study using excited state prototropism of 1-naphthol

The effect of triton X-100, sodium dodecyl sulphate and cetyl trimethyl ammonium bromide on the physical properties of dimyristoyl phosphatidyl choline liposome was studied in the sublytic concentration range of the detergent, using the different fluorescence probes 8-anilino-1-naphthalene sulphonate, 1,6-diphenylhexatriene and 1-naphthol. Depending on the nature and concentration of the detergent, and the position of the hydrophilic and hydrophobic parts of different detergents in liposomes, the fluidity and permeability of the bilayers were affected. The fluidity decreases at very low concentrations of detergent owing to an improvement in the packing density of phospholipid molecules. The extent of changes in these properties was found to be dependent on the phase state of liposomes, being greater in the solid gel phase than in the fluid liquid crystalline phase. Also, the thermotropic phase transitions of phospholipid bilayers are affected moderately by neutral surfactants and greatly by charged surfactants owing to an alteration in the water of hydration of the lipid molecules. © 1997 Elsevier Science S.A.

Journal of Photochemistry and Photobiology A: Chemistry

Effect of ionic and neutral surfactants on the properties of phospholipid vesicles: Investigation using fluorescent probes

1-Naphthol, an excited state acid, undergoes partial dissociation in the excited state in liposome membranes, giving rise to two peaks, as opposed to a single peak in water. The excited state prototropic equilibrium is affected by perturbations in the membrane system. This property can be used to probe the properties of biomembranes. In this respect, as a preliminary study, the partition coefficient of 1-naphthol in liposomes has been calculated.

Determination of the partition coefficient of 1-naphthol, an excited state acid, in DMPC membrane

Chemical Kinetics

Experimental Methods

Langmuir

Phase Transitions in Phospholipid Vesicles:  Excited State Prototropism of 1-Naphthol as a Novel Probe Concept

The Journal of Physical Chemistry

Fluorescence lifetime distributions of various phospholipids labeled with covalently bound diphenylhexatriene in the erythrocyte ghost membrane

1-Naphthol as an excited state proton transfer fluorescent probe for erythrocyte membranes

Journal	Data powered by TypesetSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Publisher	Data powered by TypesetElsevier Science B.V., Amsterdam
ISSN	13861425
Open Access	No