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Uptake of 45Ca by mitochondria of Trigonella foenum-graecum as influenced by selenium and mimosine - Detailed kinetic analyses
Kalyanaraman Lalitha
Published in
2001
PMID: 11697770
Volume: 82
   
Issue: 1-3
Pages: 217 - 229
Abstract
Mitochondria from Trigonella foenum-graecum seedlings grown independently in the presence of either selenium (0.75 ppm) or mimosine (0.1 mM) exhibited respiration-stimulated energy-dependent uptake of Ca2+. Uptake studies were carried out independently at a series of Ca2+ concentrations at two different levels: (1) 1-20 μM and (2) 25-1500 μM. Levels of uptake were 50-100% higher in the mitochondria of seedlings of both the Se and mimosine groups. Detailed kinetic analyses revealed negative cooperative effects operative during uptake of Ca2+ at 25-1500 μM given in the medium. Hill coefficients for Ca2+ uptake by the mitochondria of different groups remained unchanged (nH, 0.75). Biphasic Scatchard plots were concave upward, suggestive of two classes of binding sites. High-affinity binding sites were estimated to be 16 nmol/mg protein with dissociation constant (KCa) of 2.5 × 109 L/mol. In contrast, graphical analyses of the uptake of Ca2+ in the range 1-20 μM in the medium revealed cooperative effects of positive nature. The present study demonstrates mixed cooperative effects during Ca2+ uptake by mitochondria from seedlings of T. foenum-graecum.
About the journal
JournalBiological Trace Element Research
ISSN01634984
Open AccessNo
Concepts (31)
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    Calcium
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    MIMOSINE
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    Selenium
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    CALCIUM 45
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    MIMOSINE
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    TRIGONELLA FOENUM GRAECUM EXTRACT
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    ACTIVE TRANSPORT
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    Article
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    Drug effect
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    FENUGREEK
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    Growth, development and aging
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    Kinetics
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    Metabolism
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    Mitochondrion
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    Binding affinity
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    Binding site
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    Calcium transport
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    Dissociation constant
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    Energy
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    GERMINATION
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    MITOCHONDRIAL RESPIRATION
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    Nonhuman
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    Plant seed
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    Protein binding
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    BIOLOGICAL TRANSPORT, ACTIVE
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    CALCIUM RADIOISOTOPES
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    MIMOSINE
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    Mitochondria
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    TRIGONELLA
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    TRIGONELLA FOENUM
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    TRIGONELLA FOENUM GRAECUM