Header menu link for other important links
X
Understanding the molecular signatures in leaves and flowers by desorption electrospray ionization mass spectrometry (DESI MS) imaging
Rani Gopalakrishnan Hemalatha,
Published in
2013
Volume: 61
   
Issue: 31
Pages: 7477 - 7487
Abstract
The difference in size, shape, and chemical cues of leaves and flowers display the underlying genetic makeup and their interactions with the environment. The need to understand the molecular signatures of these fragile plant surfaces is illustrated with a model plant, Madagascar periwinkle (Catharanthus roseus (L.) G. Don). Flat, thin layer chromatographic imprints of leaves/petals were imaged using desorption electrospray ionization mass spectrometry (DESI MS), and the results were compared with electrospray ionization mass spectrometry (ESI MS) of their extracts. Tandem mass spectrometry with DESI and ESI, in conjunction with database records, confirmed the molecular species. This protocol has been extended to other plants. Implications of this study in identifying varietal differences, toxic metabolite production, changes in metabolites during growth, pest/pathogen attack, and natural stresses are shown with illustrations. The possibility to image subtle features like eye color of petals, leaf vacuole, leaf margin, and veins is demonstrated. © 2013 American Chemical Society.
About the journal
JournalJournal of Agricultural and Food Chemistry
ISSN00218561
Open AccessNo
Concepts (14)
  •  related image
    DESORPTION ELECTROSPRAY IONIZATION
  •  related image
    DESORPTION ELECTROSPRAY IONIZATION MASS SPECTROMETRIES
  •  related image
    Electrospray ionization (esi)
  •  related image
    Electrospray ionization mass spectrometry
  •  related image
    MADAGASCAR PERIWINKLES
  •  related image
    MOLECULAR SIGNATURES
  •  related image
    Surface bounds
  •  related image
    Tandem mass spectrometry
  •  related image
    Desorption
  •  related image
    Electrospray ionization
  •  related image
    Metabolites
  •  related image
    Molecular imaging
  •  related image
    Plants (botany)
  •  related image
    Mass spectrometry