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U-bent fiber optic plasmonic biosensor platform for ultrasensitive analyte detection
Bandaru R., Divagar M., Khanna S., Danny C.G., , V V R S.,
Published in Elsevier B.V.
2020
Volume: 321
   
Abstract
This study demonstrates a rapid, wash-free, dip-type plasmonic fiberoptic absorbance biosensor (P-FAB) capable of zeptomole analyte detection and 6-orders of wide dynamic range. It involves a sandwich immunoassay on a compact U-bent fiber optic probe surface that works by dipping an antibody-functionalized probe into a mixture of sample and gold nanoparticle (AuNP) label suspension. The U-bent fiberoptic probes with a high evanescent wave absorbance sensitivity allow detection of the high extinction AuNP labels measured in terms of light intensity change using a pair of LED and photodetector (PD). This simple and low-cost P-FAB gives an unprecedented detection limit of 0.17 zeptomole for human immunoglobulin G (HIgG) in 25 μL buffer in just 25 min. Further silver enhancement of AuNP labels for 5 min results in a limit of quantitation (LoQ) down to 0.17 zeptomole (∼100−150 molecules in 25 μL), making P-FAB a highly sensitive and low-cost technology for point-of-care diagnostics. © 2020 Elsevier B.V.
About the journal
JournalSensors and Actuators, B: Chemical
PublisherElsevier B.V.
ISSN09254005
Open AccessNo