The chitinase fermentation process utilizing chitin as the sole carbon source was investigated in a stirred tank bioreactor. Agitator speed of 224 rpm was found to be most suitable for cell growth as well as for chitinase production. Chitinase yield decreased rapidly at higher agitator speed, while decrease in cell yield at higher agitator speed was not rapid. Probably, mass transfer limitation was predominant in the fermentation process at lower agitator speed. Higher agitator speed appears to reduce chitinase production.

T Panda

Department Of Chemical Engineering

CHITINASE

article

bacterium culture

Bioreactor

Enzyme kinetics

enzyme synthesis

Fermentation

priority journal

TRICHODERMA

velocity

IIT Madras is a public technical and research university located in Chennai, Tamil Nadu. Founded in 1959, it is recognised as an Institute of National Importance.

IIT Madras has been ranked as the top engineering institute in India for four years in a row by the National Institutional Ranking Framework of the MHRD

It currently offers undergraduate, postgraduate and research degrees across 16 disciplines in Engineering, Sciences, Humanities and Management. About 596 faculty belonging to science and engineering departments and centres of the Institute are engaged in teaching, research and industrial consultancy.

IIT Madras

Submerged culture production of chitinase by Trichoderma harzianum in stirred tank bioreactors - The influence of agitator speed

Biochemical Engineering Journal

The generalized distance function approach is employed to obtain a suitable near optimal conditions of variables. The optimal values of variables (medium constituents, microbiological parameters, and process parameters) have been evaluated separately using single responses (either specific esterase activity or cell mass) as per central-composite-design and multi-responses following generalized distance function approach. The optimal conditions (medium composition (g l-1): dextrose, 13.43; peptone, 7.285; yeast extract, 2.55; and malt extract, 1.695; microbiological parameters: slant age, 39.9 h; inoculum age, 9.6 h; and number of cells, 1.49 × 108 numbers ml-1; process conditions: temperature, 29.9 °C; and pH, 6.2) obtained by generalized distance approach can be considered as a 'near optimal' solution of interactive multi-response systems of intracellular esterase synthesis by Saccharomyces cerevisiae. © 2007 Elsevier Ltd. All rights reserved.

Bioresource Technology

Critical analysis of application of generalized distance function for optimization of important variables for esterase synthesis by Saccharomyces cerevisiae

Chitin, a homopolymer of N-acetylglucosamine, is obtained from a variety of sources. They form the structural component of fungal cell wall and plants. They are commercially obtained from shrimp and crab shell waste from the fishing industry. Recent advances in understanding the structure and properties of chitin and its derivatives has opened a lot of new avenues for its applications. Improvements in the properties of chitin for a particular application can be easily brought about by chemical modifications. The applicability of chitin in many areas and its easy manipulation has resulted in a considerable amount of research being done on the possible applications of chitinase.

Bioprocess Engineering

Studies on applications of chitin and its derivatives

A kinetic analysis and optimization of reaction conditions for the enzymatic hydrolysis of chitin using chitinase produced by Trichoderma harzianum NCIM 1185 was carried out. Swollen chitin was used as the substrate for chitinase. The central composite design was followed for this optimization. The required volume ratio of the major reactants for maximum hydrolysis was determined. The pH and temperature optima were found to be 4.75 and 47 °C respectively. K(m) and V(max) for this enzyme were 4.643 kg/m3 and 0.1542 U respectively.

Parameters optimization of chitin hydrolysis by Trichoderma harzianum chitinase under assay conditions

Statistical design was used to determine the optimal levels of medium components, the optimal initial pH of the enzyme production medium, the temperature of fermentation, age of the organism in the slant growth and the age of the inoculum for the production of chitinase in shake flask fermentations. The use of high concentrations of chitin and ammonium sulphate and exclusion of peptone and urea from the medium resulted in the production of higher level of the enzyme. The optimal concentrations of the medium components were 12.5 kg/m3 and 4.2 kg/m3 for the chitin and ammonium sulphate respectively. The effect of the addition of peptone and urea to the optimized medium was studied. The optimal values of initial pH and temperature were 5.6 and 28 °C respectively. The optimal age of the slant and the inoculum were found to be 105 h and 43 h respectively. The highest level of chitinase before optimization of the above variables was 0.054 U which was maximized to the level of 0.197 U.

Optimization of carbon and nitrogen sources in the medium and environmental factors for enhanced production of chitinase by trichoderma harzianum

Journal of Chemical Technology & Biotechnology

Metabolic and biosynthetic responses of hybridomas subjected to hydrodynamic stress

Biotechnology and Bioengineering

Dependence of mycelial morphology on impeller type and agitation intensity

Journal	Biochemical Engineering Journal
ISSN	1369703X
Open Access	No