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Studies on varying n-alkanethiol chain lengths on a gold coated surface and their effect on antibody-antigen binding efficiency
Priyanka Bhadra, Mohammed S. Shajahan, ,
Published in Royal Society of Chemistry
2015
Volume: 5
   
Issue: 98
Pages: 80480 - 80487
Abstract
Self-assembled monolayer (SAM) of n-alkanethiols of different chain lengths (n = 2, 3, 6, 11, 16) on a gold surface are used to immobilize antibodies which in turn bind to a antigen. The antibody and antigen used in this study have similar molecular weights i.e. ∼150 kDa. The antibody [1.5 μg cm-2] immobilized varied with the surface packing density of the SAM of carboxylic acid-terminated n-alkanethiols of different lengths. In comparison, the efficiency of antibody immobilization was lowest on the loosely packed SAM of n-alkanethiols (n ≤ 3) and the highest on the densely packed SAM of n-alkanethiols (n ≥ 11). However, increased immobilization of antibodies with increasing chain length of the n-alkanethiols [n > 11], did not result in a corresponding increase in antigen binding. An attempt to explain this phenomenon based on packing density and an orientation of the captured antibody is presented. © 2015 The Royal Society of Chemistry.
About the journal
JournalData powered by TypesetRSC Advances
PublisherData powered by TypesetRoyal Society of Chemistry
ISSN20462069
Open AccessNo
Concepts (15)
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    Antigens
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    Chain length
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    Chains
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    Gold
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    Gold coatings
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    Self assembled monolayers
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    Alkanethiols
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    Antibody immobilization
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    Antibody-antigen
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    Antigen binding
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    Gold surfaces
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    GOLD-COATED SURFACE
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    N-ALKANETHIOLS
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    Packing density
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    Antibodies