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Studies on encapsulation of bovine serum albumin, lysozyme and insulin through coaxial electrospinning
, Anant Raheja, A Agarwal, T.S Natarajan,
Published in American Scientific Publishers
2013
Volume: 3
   
Issue: 6
Pages: 669 - 672
Abstract
A single step method for encapsulation of clinically relevant proteins viz., Bovine Serum Albumin (BSA), Lysozyme and Insulin molecules, inside core-sheath structured polymer fibres using coaxial electrospinning technique, is described. Transmission electron microscopic pictures show the formation of core-sheath structure. The secondary structure of the proteins was characterized through CD spectroscopy. The secondary conformation was retained with a minor decrease in β-conformation and an increase in the random and turn conformations after coaxial electrospinning. This method of encapsulation is simple and requires little or no harsh treatment of sensitive biomolecules like insulin. Thus, the core-sheath structure of the fibres might apparently help protect the biomolecules during and after electrospinning, in addition to providing a high interactive surface area for controlled release. This scheme will be suitable for applications in cell culture and tissue regeneration. © 2013 American Scientific Publishers, All rights reserved.
About the journal
JournalData powered by TypesetJournal of Biomaterials and Tissue Engineering
PublisherData powered by TypesetAmerican Scientific Publishers
ISSN21579083
Open AccessNo
Concepts (12)
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    Bovine serum albumin
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    Insulin
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    Lysozyme
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    Article
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    Circular dichroism
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    Electrospinning
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    Encapsulation
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    Phase separation
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    Protein analysis
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    Protein structure
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    Tissue regeneration
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    Transmission electron microscopy