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SNAP-Tag Technology: A Promising Tool for Ex Vivo Immunophenotyping
Swati Choudhary,
Published in Springer International Publishing
2017
PMID: 28164252
Volume: 21
   
Issue: 3
Pages: 315 - 326
Abstract
Background: SNAP-tag, a self-labeling protein tag, is commonly used for in vitro and in vivo analysis of bound target proteins. We report the first evidence that SNAP-tag could be used for ex vivo detection of enriched biological markers. Methods: Proof of concept was established for target c-kit receptor, a pathological and diagnostic marker for a variety of cancers. SNAP-tag conjugates with stem-cell factor (SCF) fusion proteins were designed and their binding and specificity was validated in vitro using flow cytometry and immunostaining. Results: Ex vivo diagnostic application of the fusion protein was demonstrated in comparison with anti-c-kit antibody for peripheral blood samples from leukemia patients and colorectal tissue specimens. © 2017, Springer International Publishing Switzerland.
About the journal
JournalData powered by TypesetMolecular Diagnosis and Therapy
PublisherData powered by TypesetSpringer International Publishing
ISSN11771062
Open AccessNo
Concepts (24)
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    Hybrid protein
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    METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE
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    PARAFFIN
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    Stem cell factor
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    Stem cell factor receptor
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    ACUTE MYELOID LEUKEMIA
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    Cell line
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    Chemistry
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    COLORECTAL TUMOR
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    Genetics
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    Human
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    Immunology
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    IMMUNOPHENOTYPING
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    Metabolism
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    Pathology
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    Procedures
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    COLORECTAL NEOPLASMS
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    Humans
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    IMMUNOPHENOTYPING
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    LEUKEMIA, MYELOID, ACUTE
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    O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE
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    PARAFFIN EMBEDDING
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    Proto-oncogene proteins c-kit
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    Recombinant fusion proteins