The self-directing optimization technique was employed to determine the optimal conditions for β-1,3-glucanase production by Trichoderma harzianum in batch culture. A maximum β-1,3-glucanase production of 0.910 U was obtained at a pH (controlled) of 4.9, an aeration rate of 0.9 1/(l)(min), and an agitator speed of 220 rev/min which were found to be the most suitable.

T Panda

Department Of Chemical Engineering

Kalaimahan Théodore

1,3 BETA GLUCANASE

article

controlled study

enzyme synthesis

HYPHOMYCETES

nonhuman

priority journal

IIT Madras is a public technical and research university located in Chennai, Tamil Nadu. Founded in 1959, it is recognised as an Institute of National Importance.

IIT Madras has been ranked as the top engineering institute in India for four years in a row by the National Institutional Ranking Framework of the MHRD

It currently offers undergraduate, postgraduate and research degrees across 16 disciplines in Engineering, Sciences, Humanities and Management. About 596 faculty belonging to science and engineering departments and centres of the Institute are engaged in teaching, research and industrial consultancy.

IIT Madras

Bioprocess Engineering

Response surface methods were used to determine the optimum concentration of medium nutrients for extracellular β-1,3-glucanase production by Trichoderma harzianum in shakeflask culture. A Plackett-Burman design was used to screen the important variables which were then grouped and studied by a central composite design. The optimum levels of the medium constituents were determined by the complex algorithm of Box. © 1994 Chapman & Hall, London.

Biotechnology Techniques

Statistical optimization of production medium for β-1,3-glucanase synthesis by Trichoderma harzianum

Surface and submerged culture studies were carried out for the production of extracellular β-1,3-glucanase and carboxymethylcellulase from Trichoderma harzianum, NCIM 1185. The different parameters which influence the enzyme production, viz., spore age in the slant growth, seed age, and pH (initial) of the production medium have been optimized. The ranges of these parameters studied were: Spore age of T. harzianum between 2 and 10 days, seed age between 12 h and 48 h, and pH (initial) between 3 and 7. The other conditions of fermentation were: Temperature 30°C, gyratory shaker speed 160 rev. min-1. It was observed that the activities of β-1,3-glucanase and carboxymethylcellulase varied during the entire fermentation cycle which had a distinct effect on protoplast generation from T. reesei mycelium. The optimum values of the above-mentioned parameters for lytic enzymes production were: Spore age of T. harzianum in the slant growth 5 days, seed age 36 h, and pH (initial) 5.0 for both surface and submerged culture processes. © 1994 Springer-Verlag.

Production of β-1,3-glucanase from Trichoderma harzianum in surface and submerged culture processes and its role on protoplast generation from Trichoderma reesei mycelium

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Self-directing optimization of β-1,3-glucanase production by Trichoderma harzianum in batch culture

Journal	Bioprocess Engineering
ISSN	0178515X
Open Access	No