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Self-crosslinked oxidized alginate/gelatin hydrogel as injectable, adhesive biomimetic scaffolds for cartilage regeneration
Athipettah Jayakrishnan
Published in Elsevier Ltd
2014
PMID: 24811827
Volume: 10
   
Issue: 8
Pages: 3650 - 3663
Abstract
Biopolymeric hydrogels that mimic the properties of extracellular matrix have great potential in promoting cellular migration and proliferation for tissue regeneration. The authors reported earlier that rapidly gelling, biodegradable, injectable hydrogels can be prepared by self-crosslinking of periodate oxidized alginate and gelatin in the presence of borax, without using any toxic crosslinking agents. The present paper investigates the suitability of this hydrogel as a minimally invasive injectable, cell-attractive and adhesive scaffold for cartilage tissue engineering for the treatment of osteoarthritis. Time and frequency sweep rheology analysis confirmed gel formation within 20 s. The hydrogel integrated well with the cartilage tissue, with a burst pressure of 70 ± 3 mmHg, indicating its adhesive nature. Hydrogel induced negligible inflammatory and oxidative stress responses, a prerequisite for the management and treatment of osteoarthritis. Scanning electron microscopy images of primary murine chondrocytes encapsulated within the matrix revealed attachment of cells onto the hydrogel matrix. Chondrocytes demonstrated viability, proliferation and migration within the matrix, while maintaining their phenotype, as seen by expression of collagen type II and aggrecan, and functionality, as seen by enhanced glycosoaminoglycan (GAG) deposition with time. DNA content and GAG deposition of chondrocytes within the matrix can be tuned by incorporation of bioactive signaling molecules such as dexamethasone, chondroitin sulphate, platelet derived growth factor (PDGF-BB) and combination of these three agents. The results suggest that self-crosslinked oxidized alginate/gelatin hydrogel may be a promising injectable, cell-attracting adhesive matrix for neo-cartilage formation in the management and treatment of osteoarthritis. © 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
About the journal
JournalData powered by TypesetActa Biomaterialia
PublisherData powered by TypesetElsevier Ltd
ISSN17427061
Open AccessNo
Concepts (73)
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    AGGRECAN
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    Alginic acid
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    Biomimetic material
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    BORATE SODIUM
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    CHONDROITIN SULFATE
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    COLLAGEN TYPE 2
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    Dexamethasone
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    DNA
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    Gelatin
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    GLYCOSAMINOGLYCAN
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    PLATELET DERIVED GROWTH FACTOR BB
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    CROSS LINKING REAGENT
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    Glucuronic acid
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    Hexuronic acid
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    Hydrogel
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    Tissue adhesive
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    Animal cell
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    Article
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    Bone regeneration
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    Cartilage
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    CARTILAGE CELL
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    Cartilage regeneration
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    Cell adhesion
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    Cell migration
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    Cell proliferation
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    Cell viability
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    Cross linking
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    Dna content
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    Flow kinetics
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    Mouse
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    Nonhuman
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    Osteoarthritis
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    Oxidative stress
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    Priority journal
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    Protein expression
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    Scanning electron microscopy
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    Tissue engineering
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    Adhesion
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    Animal
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    C57bl mouse
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    Cell culture
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    Chemistry
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    Cytology
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    Device failure analysis
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    Devices
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    Growth, development and aging
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    HYALINE CARTILAGE
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    Injection
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    Materials testing
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    Oxidation reduction reaction
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    Physiology
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    Prosthesis
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    Regeneration
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    Tissue scaffold
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    MURINAE
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    ADHESIVENESS
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    Alginates
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    Animals
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    Biomimetic materials
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    Cells, cultured
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    Chondrocytes
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    Cross-linking reagents
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    Equipment failure analysis
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    Hexuronic acids
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    HYALINE CARTILAGE
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    Hydrogels
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    Injections
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    Mice
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    Mice, inbred c57bl
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    Oxidation-reduction
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    Prosthesis design
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    TISSUE ADHESIVES
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    Tissue scaffolds