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Selenium-mediated differential response of β-glucosidase and β- galactosidase of germinating Trigonella foenum-graecum
Kalyanaraman Lalitha
Published in Humana Press
1998
PMID: 9845479
Volume: 64
   
Issue: 1-3
Pages: 247 - 258
Abstract
β-Glucosidase and β-galactosidase activity profile tested in different seeds during 24 h germination revealed reasonably high levels of activity in Vigna radiata, Cicer arietinum, and Trigonella foenum-graecum. In all seeds tested, β-galactosidase activity was, in general, higher than that of β- glucosidase. T. foenum-graecum seedlings exhibited maximal total and specific activities for both the enzymes during 72 h germination. Se supplementation as Na2SeO3 up to 0.75 ppm was found to be beneficial to growth and revealed selective enhancement of β-galactosidase activity by 40% at 0.5 ppm Se. The activities of both the enzymes drastically decreased at 1.0 ppm level of Se supplementation. On the contrary, addition of Na2SeO3 in vitro up to 1 ppm to the enzyme extracts did not influence these activities. Hydrolytic rates of β-glucosidase in both control and Se-supplemented groups were enhanced by 20% with 0.05 M glycerol in the medium and 30% at 0.1 M glycerol. The rates were marginally higher in Se-supplemented seedlings than the controls, irrespective of added glycerol in the medium. In contrast, hydrolysis by β- galactosidase showed a trend of decrease in Se-supplemented seedlings compared to the control, when glycerol was present in the medium. Addition of Se in vitro in the assay medium showed no difference in the hydrolytic rate by β-galactosidase when compared to control, while the activity of β- glucosidase declined by 50%. Se-grown seedlings showed an enhancement of transglucosidation rate by 40% in the presence of 0.1 M glycerol. The study reveals a differential response to Se among the β-galactosidase and β- glucosidase of T. foenum-graecum with increase in the levels of β- galactosidase activity.
About the journal
JournalBiological Trace Element Research
PublisherHumana Press
ISSN01634984
Open AccessNo
Concepts (21)
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    Beta galactosidase
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    BETA GLUCOSIDASE
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    Selenium
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    Trace element
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    TRIGONELLA FOENUM GRAECUM EXTRACT
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    Article
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    Bacterial growth
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    Diet supplementation
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    Energy metabolism
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    Enzyme activity
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    Enzyme mechanism
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    Enzyme specificity
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    GERMINATION
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    Hydrolysis
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    Nonhuman
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    Plant seed
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    Bacteria (microorganisms)
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    CICER ARIETINUM
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    TRIGONELLA FOENUM
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    TRIGONELLA FOENUM GRAECUM
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    VIGNA RADIATA