We search for CP violation in the singly-Cabibbo-suppressed decay D0→K+K-π+π- using data corresponding to an integrated luminosity of 988 fb-1 collected by the Belle detector at the KEKB e+e- collider. We measure a set of five kinematically dependent CP asymmetries, of which four asymmetries are measured for the first time. The set of asymmetry measurements can be sensitive to CP violation via interference between the different partial-wave contributions to the decay and performed on other pseudoscalar decays. We find no evidence of CP violation. © 2019 authors. Published by the American Physical Society. Published by the American Physical Society under the terms of the »https://creativecommons.org/licenses/by/4.0/» Creative Commons Attribution 4.0 International license. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI. Funded by SCOAP3.