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Release of periplasmic penicillin amidase from Escherichia coli by chloroform shock
K. Jagannadha Rao,
Published in Springer Verlag
Volume: 14
Issue: 2
Pages: 101 - 103
Penicillin amidase is a periplasmic enzyme in Escherichia coli. Conventionally, the periplasmic enzymes are released into the medium by osmotic shock which is tedious involving a number of centrifugation steps. The present communication deals with a simple technique for the release of penicillin amidase by chloroform shock. Experimental findings show that the periplasmic penicillin amidase does not show any variation by the chloroform treatment. This analysis was also extended to the E. coli cells grown at various concentrations of phenylacetic acid, optimal concentration of phenylacetic acid plus glucose and lactic acid.
About the journal
JournalData powered by TypesetBioprocess Engineering
PublisherData powered by TypesetSpringer Verlag
Open AccessNo
Concepts (9)
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    Penicillin amidase
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    Controlled study
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    Enzyme release
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    Escherichia coli
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    Priority journal
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