In this study, an effort has been made to increase the observation volume of fluorescent analytes using a cavity shaped and reflective walled sample compartment with a fiber-optic spectrofluorometer setup. Aluminium (Al) is used as a coating material due to its uniform reflectance over UV–Vis range. The converging nature of the sample compartment along with the reflective coating helped to enhance the analyte volume that is being irradiated by the excitation radiation, consequently the fluorescence intensity increases. A co-axial fiber is used to project excitation radiation and to collect fluorescence emission; an oblique angle of 35° w.r.t. normal of sample surface is found to be optimal to obtain clean fluorescence spectra even without the use of a cut-off filter. The accuracy of fluorescence data obtained using the proposed method is validated by comparing with the data from a commercial table-top spectrofluorometer. With fiber-optic setup, this method is a cost-effective and a simple approach towards sensitive fluorescence detection. Further, it is known that mirror coated quartz cuvettes have been used in table-top commercial spectrofluorometer to enhance fluorescence emission; but the effect of spectral response of the mirror coated material on emission spectral profile of analyte is not reported. In this article the spectral response of mirror coated material is studied and subsequently a correction factor file is generated. © 2019 Elsevier Ltd