We report, a nanosecond pulse laser activated photoporation for high efficient intracellular delivery using nanocorrugated mushroom shape gold nanoparticles (nm-AuNPs). When pulse laser ex-pouse on cell membrane with nm-AuNPs, plasmonic nanobubbles (PNB) can generate and produce very strong fluid flow resulting to create transient membrane pores to deliver cargos into cells. Our photoporation technique can achieve 88% intracellular PI dye uptake of CL1-0 cells and close to 98% cell viability at 480 nm wavelength. Successful plasmid delivery was performed with 82% transfec-tion efficiency and 98% cell viability at 897 nm (nm-AuNPs have absorption at 480 nm and 897 nm) wavelength.