Header menu link for other important links
X
Proton-gated ion channels in mouse bone marrow stromal cells
Published in
2012
PMID: 22677706
Volume: 9
   
Issue: 2
Pages: 59 - 68
Abstract
A variety of ion channels like acid sensing ion channels (ASICs) and several members of the transient receptor potential (TRP) cation channel family are known to be activated by protons. The present study describes proton-gated current in mouse bone marrow stromal cells (BMSCs), by using whole cell patch clamp. Rapid application of extracellular solution of pH≤6.5, evoked slow inactivating current with mean peak value of 328±31pA, (n=25) at pH 5.0. The reversal potential was close to the theoretical Na+ equilibrium potential, indicating that majority of the current is mediated by Na+ and partially carried by Ca2+ as revealed by ion substitution experiments and Ca2+ imaging. ASICs blocker amiloride (1mM) and nonselective cation channel blocker flufenamic acid (0.3mM) reduced the current amplitudes by 36±5% (n=10) and 39±7% (n=14) respectively. Co-application of flufenamic acid and amiloride further decreased the current by 70±7% (n=7). However, capsazepine, SKF 96365 and ruthenium red had no effect. 10mM of Ca2+ and 2mM of La3+ inhibited the current by 39±6% (n=5) and 46±6% (n=4) respectively. Zn2+ (300μM) and Gd3+ (500μM) had no effect on the current amplitude. Low pH mediated cell death was completely inhibited by co-application of La3+ and amiloride. Reverse Transcriptase-PCR detected expression of mRNAs of ASICs and TRP family. In summary, our results demonstrate the functional expression of low pH-activated ion channels in mouse BMSCs. © 2012 Elsevier B.V.
About the journal
JournalStem Cell Research
ISSN18735061
Open AccessYes
Concepts (44)
  •  related image
    ACID SENSING ION CHANNEL
  •  related image
    AMILORIDE
  •  related image
    Calcium ion
  •  related image
    CAPSAZEPINE
  •  related image
    FLUFENAMIC ACID
  •  related image
    Ion channel
  •  related image
    Messenger rna
  •  related image
    PROTON GATED ION CHANNEL
  •  related image
    Sodium ion
  •  related image
    Unclassified drug
  •  related image
    VANILLOID RECEPTOR
  •  related image
    Animal cell
  •  related image
    Article
  •  related image
    BONE MARROW STROMAL CELL
  •  related image
    Calcium transport
  •  related image
    Cell death
  •  related image
    Concentration (parameters)
  •  related image
    EXTRACELLULAR CALCIUM
  •  related image
    Gene expression
  •  related image
    ION CURRENT
  •  related image
    ION PERMEABILITY
  •  related image
    Mouse
  •  related image
    Nonhuman
  •  related image
    Nucleotide sequence
  •  related image
    pH
  •  related image
    Priority journal
  •  related image
    Protein expression
  •  related image
    STROMA CELL
  •  related image
    ACID SENSING ION CHANNEL BLOCKERS
  •  related image
    ACID SENSING ION CHANNELS
  •  related image
    Animals
  •  related image
    Calcium
  •  related image
    Cell membrane permeability
  •  related image
    Extracellular space
  •  related image
    Hydrogen-ion concentration
  •  related image
    Ion channel gating
  •  related image
    Ions
  •  related image
    Mesenchymal stromal cells
  •  related image
    Mice
  •  related image
    Protons
  •  related image
    Reverse transcriptase polymerase chain reaction
  •  related image
    Rna, messenger
  •  related image
    Sodium
  •  related image
    TRANSIENT RECEPTOR POTENTIAL CHANNELS