Header menu link for other important links
X
Production of xylooligosaccharides in SSF by Bacillus subtilis KCX006 producing β-xylosidase-free endo-xylanase and multiple xylan debranching enzymes
Published in Taylor and Francis Inc.
2016
PMID: 25310011
Volume: 46
   
Issue: 1
Pages: 49 - 55
Abstract
Xylanase and xylooligosaccharides (XOS) are employed in food and feed industries. Though xylanase production from lignocellulosic materials (LCMs) by solid-state fermentation (SSF) is well known, the XOS formed during growth is not recovered due to its conversion to xylose by β-xylosidase and subsequent bacterial metabolism. A new strain, Bacillus subtilis KCX006, was exceptionally found to synthesize β-xylosidase-free endo-xylanase and multiple xylan debranching enzymes constitutively in the presence of LCMs. Absence of β-xylosidase resulted in accumulation of XOS during growth of KCX006 on LCMs. Therefore, this strain was used for simultaneous production of xylanase and XOS from agro-residues in solid-state fermentation (SSF). Partial purification of XOS from culture supernatant using activated charcoal followed by high-performance liquid chromatography (HPLC) analysis showed xylobiose to xylotetraose formed as the major products. Among various LCM substrates, wheat bran and groundnut oil-cake supported highest xylanase and XOS production at 2158 IU/gdw and 24.92 mg/gdw, respectively. The levels of xylanase and XOS were improved by 1.5-fold (3102 IU/gdw) and 1.9-fold (48 mg/gdw), respectively, by optimization of culture conditions. Copyright © 2016 Taylor & Francis Group, LLC.
About the journal
JournalData powered by TypesetPreparative Biochemistry and Biotechnology
PublisherData powered by TypesetTaylor and Francis Inc.
ISSN10826068
Open AccessNo
Concepts (20)
  •  related image
    EXO-1,4-BETA-D-XYLOSIDASE
  •  related image
    Glucuronic acid
  •  related image
    Oligosaccharide
  •  related image
    XYLAN 1,4 BETA XYLOSIDASE
  •  related image
    XYLOOLIGOSACCHARIDE
  •  related image
    Bacillus subtilis
  •  related image
    Enzyme specificity
  •  related image
    Fermentation
  •  related image
    High performance liquid chromatography
  •  related image
    Metabolism
  •  related image
    Microbiology
  •  related image
    pH
  •  related image
    Temperature
  •  related image
    Chromatography, high pressure liquid
  •  related image
    GLUCURONATES
  •  related image
    Hydrogen-ion concentration
  •  related image
    Oligosaccharides
  •  related image
    Soil microbiology
  •  related image
    Substrate specificity
  •  related image
    XYLOSIDASES