Recent advancements in biosensor design and fabrication for invasive and noninvasive monitoring of cardiovascular diseases (CVDs) have been demanding. This can be attributed to the simultaneous advancement in polymer chemistry, technology relating to microelectronics development, interdisciplinary research encompassing interface and behaviour of biological tissue and nonbiological material interaction to name a few. To design an invasive or noninvasive biosensor system for CVD application, polymers used for various parts and components of the biosensor play a crucial role in the success and in vivo performance of the system along with maintaining high sensitivity and accuracy of the biosensor. In this perspective, this review summarizes the importance of polymeric materials in the next-generation diagnostic approaches for cardiovascular health care. The review discusses the recent advancements of polymeric materials in the design and fabrication of biosensing agents, substrates and components, highlighting recent biosensor platforms in real-time biosensing cardiovascular health and disease application. © 2019 Elsevier Inc.