The combined effect of pH and temperature on carboxymethyl cellulase from two intergeneric fusants (M 14 and M 62) of Trichoderma reesei QM 9414/Saccharomyces cerevisiae NCIM 3288 was studied using response surface methodology. A central composite design for two variables was employed for the optimization studies. This study was compared with similar studies carried out with Trichoderma reesei QM 9414. The optimal pH and temperature for the enzymes derived from these organisms were: for the fusant M 14 - pH 5.7 and 41.7°C, for the fusant M 62 - pH 5.3 and 43°C, and for Trichoderma reesei QM 9414 - pH 4.31 and 38.3°C.

T Panda

Department Of Chemical Engineering

R. Srinivas

CELLULASE

GLUCAN SYNTHASE

article

enzyme activity

Enzyme Stability

enzyme synthesis

methodology

Saccharomyces cerevisiae

Temperature

thermostability

TRICHODERMA REESEI

HYPOCREA JECORINA

TRICHODERMA

IIT Madras is a public technical and research university located in Chennai, Tamil Nadu. Founded in 1959, it is recognised as an Institute of National Importance.

IIT Madras has been ranked as the top engineering institute in India for four years in a row by the National Institutional Ranking Framework of the MHRD

It currently offers undergraduate, postgraduate and research degrees across 16 disciplines in Engineering, Sciences, Humanities and Management. About 596 faculty belonging to science and engineering departments and centres of the Institute are engaged in teaching, research and industrial consultancy.

IIT Madras

Journal of Industrial Microbiology and Biotechnology

Single-stage direct bioconversion of cellulosic materials to ethanol, under almost the same fermentation conditions applied to the Saccharomyces cerevisiae-ethanol process, was attempted using intergeneric fusants of Trichoderma reesei QM 9414/S. cerevisiae NCIM 3288. Optimization of medium constituents was made using the Box-Behnken design method. The combined effect of pH and temperature on the fermentation was analyzed by the central composite design method. Under optimal conditions the fusant produced 0.17 g l-1 ethanol in 30 h. © 1995.

Enzyme and Microbial Technology

Direct conversion of cellulosic material to ethanol by the intergeneric fusant Trichoderma reesei QM 9414/Saccharomyces cerevisiae NCIM 3288

An attempt to produce ethanol from a cellulosic substrate by a single-stage process using intergeneric hybrids obtained from Trichoderma reesei QM 9414 and Saccharomyces cerevisiae NCIM 3288 fusants is described. Two hundred one fused strains were isolated using two different combinations of hybridization, followed by polyethylene glycol treatment of protoplasts derived from parent and mutant strains. Some fusants were either diploids or heterokaryons, as verified by a protoplast fused system obtained by auxotrophic mutants derived from T. reesei QM 9414 and S. cerevisiae NCIM 3288. Of the 201 fusants, 170 were found to be relatively stable. Two fusants (M14 and M62) showed the highest synthesis of ethanol from filter paper cellulose, producing 0.0149 and 0.0191 g l-1, respectively. © 1994.

Intergeneric hybridization of Trichoderma reesei QM9414 and Saccharomyces cerevisiae NCIM 3288 by protoplast fusion

Biotechnology and Bioengineering

pH-Dependent enzyme deactivation models

Methods in Enzymology Biomass Part A: Cellulose and Hemicellulose

Methods for measuring cellulase activities

Biotechnology and Genetic Engineering Reviews

The Properties of Fungal and Bacterial Cellulases with Comment on their Production and Application

pH and thermal stability studies of carboxymethyl cellulase from intergeneric fusants of Trichoderma reesei/Saccharomyces cerevisiae

Journal	Journal of Industrial Microbiology and Biotechnology
ISSN	13675435
Open Access	No