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Optimization of batch fermentation conditions for dextran production
R. S. Karthikeyan, Anantharanganathan Baradarajan
Published in Springer Verlag
1996
Volume: 15
   
Issue: 5
Pages: 247 - 251
Abstract
The nutrient medium (containing sucrose, yeast extract and K2HPO4), temperature and initial pH conditions were optimised for batch dextran production in shake flask fermentations using a strain of Leuconostoc mesenteroides NRRL B 512 (F). A 25-1 fractional factorial central composite experimental design was attempted. Multistage Monte Carlo optimization program was used to maximize the multiple regression equation obtained. The optimal values of tested variables for maximal dextran production were found to be: sucrose, 300 g/l; yeast extract, 10 g/l; K2HPO4, 30 g/l; temperature, 23°C and initial pH 8.3 with a predicted dextran yield of 154 g/l.
About the journal
JournalData powered by TypesetBioprocess Engineering
PublisherData powered by TypesetSpringer Verlag
ISSN0178515X
Open AccessNo
Concepts (15)
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    Dextran
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    Article
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    Bacterium culture
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    Bioreactor
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    Controlled study
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    Culture medium
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    Engineering
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    Fermentation
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    LEUCONOSTOC
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    Nonhuman
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    Priority journal
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    Yeast
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    Bacteria (microorganisms)
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    LEUCONOSTOC
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    LEUCONOSTOC MESENTEROIDES