We show that plasmonic chirality in single gold nanorod (GNR) aggregates leads to the rotation of polarization of the scattered light. 3D glasses in conjunction with linearly polarized dark field scattering microspectroscopy were used to study the chirality of single GNR aggregates. Using this hetero-polarizer setup, we not only detect but also quantify their chirality. A polar mapping strategy was used for providing direct evidence for the emergence of light of different polarization angles when chiral GNR aggregates were excited with circularly polarized light of different handedness. Further, we have developed a methodology to eliminate fluctuations in the scattering intensity by averaging and normalizing the data. This allows calculation of plasmonic circular dichroism scattering spectra with high accuracy. © 2014 AIP Publishing LLC.