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Nanosecond laser ablation enhances cellular infiltration in a hybrid tissue scaffold
Sarvepalli Jahnavi, N. Arthi, G. S. Bhuvaneshwar,
Published in Elsevier Ltd
2017
PMID: 28532021
Volume: 77
   
Pages: 190 - 201
Abstract

Hybrid tissue engineered (HTE) scaffolds constituting polymeric nanofibers and biological tissues have attractive bio-mechanical properties. However, they suffer from small pore size due to dense overlapping nanofibers resulting in poor cellular infiltration. In this study, using nanosecond (ns) laser, we fabricated micro-scale features on Polycaprolactone (PCL)-Chitosan (CH) nanofiber layered bovine pericardium based Bio-Hybrid scaffold to achieve enhanced cellular adhesion and infiltration. The laser energy parameters such as fluence of 25 J/cm2, 0.1 mm instep and 15 mark time were optimized to get structured microchannels on the Bio-Hybrid scaffolds. Laser irradiation time of 40 μs along with these parameters resulted in microchannel width of ~ 50 μm and spacing of ~ 35 μm between adjacent lines. The biochemical, thermal, hydrophilic and uniaxial mechanical properties of the Bio-Hybrid scaffolds remained comparable after laser ablation reflecting extracellular matrix (ECM) stability. Human umbilical cord mesenchymal stem cells and mouse cardiac fibroblasts seeded on these laser-ablated Bio-Hybrid scaffolds exhibited biocompatibility and increased cellular adhesion in microchannels when compared to non-ablated Bio-Hybrid scaffolds. These findings suggest the feasibility to selectively ablate polymer layer in the HTE scaffolds without affecting their bio-mechanical properties and also describe a new approach to enhance cellular infiltration in the HTE scaffolds. © 2017 Elsevier B.V.

About the journal
JournalData powered by TypesetMaterials Science and Engineering C
PublisherData powered by TypesetElsevier Ltd
ISSN09284931
Open AccessNo
Concepts (39)
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    Ablation
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    Adhesion
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    Biocompatibility
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    Biomechanics
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    Cell culture
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    Laser ablation
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    Mammals
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    Mechanical properties
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    Microchannels
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    Nanofibers
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    Polycaprolactone
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    Polymers
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    Pore size
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    Stem cells
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    Tissue
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    Tissue engineering
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    BIO-HYBRIDS
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    Cellular infiltration
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    Extracellular matrices
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    HYBRID TISSUE ENGINEERING (HTE)
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    Mesenchymal stem cell
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    NANOSECOND LASER ABLATION
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    Nanosecond lasers
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    Structured microchannels
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    Scaffolds (biology)
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    Nanofiber
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    Polyester
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    Animal
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    Bovine
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    Human
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    Low level laser therapy
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    Tissue scaffold
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    Animals
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    Cattle
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    Cells, cultured
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    Humans
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    Laser therapy
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    Polyesters
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    Tissue scaffolds