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Miniaturization of EISCAP sensor for triglyceride detection
Hareesh Vemulachedu, ,
Published in
2009
PMID: 18649048
Volume: 20
   
Issue: SUPPL. 1
Pages: S229 - S234
Abstract
In this paper we discuss the fabrication and characterization of miniaturized triglyceride biosensors on crystalline silicon and porous silicon (PS) substrates. The sensors are miniaturized Electrolyte Insulator Semiconductor Capacitors (mini-EISCAPs), which primarily sense the pH variation of the electrolyte used. The lipase enzyme, which catalyses the hydrolysis of triglycerides, was immobilized on the sensor surface. Triglyceride solutions introduced into the enzyme immobilized sensor produced butyric acid which causes the change in pH of the electrolyte. Miniaturized EISCAP sensors were fabricated using bulk micromachining technique and have silicon nitride as the pH sensitive dielectric layer. The sensors are cubical pits of dimensions 1,500 μm × 1,500 μm × 100 μm which can hold an electrolyte volume of 0.1 μl. The pH changes in the solution can be sensed through the EISCAP sensors by monitoring the flatband voltage shift in the Capacitance-Voltage (C-V) characteristics taken during the course of the reaction. The reaction rate is found to be quite high in the miniature cells when compared to the sensors of bigger dimensions. © 2008 Springer Science+Business Media, LLC.
About the journal
JournalJournal of Materials Science: Materials in Medicine
ISSN09574530
Open AccessNo
Concepts (50)
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    BULK- MICROMACHINING
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    BUTYRIC ACIDS
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    Capacitance-voltage characteristics
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    CRYSTALLINE SILICONS
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    DIELECTRIC LAYER
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    ELECTROLYTE INSULATOR SEMICONDUCTORS
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    FLAT-BAND VOLTAGE SHIFT
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    LIPASE ENZYME
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    Ph change
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    Ph sensitive
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    PH VARIATION
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    SENSOR SURFACES
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    Biosensors
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    Capacitance
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    Electrolysis
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    Electrolytes
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    Enzymes
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    Fatty acids
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    Porous silicon
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    Reaction rates
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    Silicon nitride
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    Electrolytic capacitors
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    Butyric acid
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    Electrolyte
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    Silicon
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    Triacylglycerol
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    TRIACYLGLYCEROL LIPASE
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    Biosensor
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    Catalysis
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    Chemical reaction
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    Conference paper
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    Crystal
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    Dielectric constant
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    Electric potential
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    Enzyme immobilization
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    Hydrolysis
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    pH
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    Priority journal
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    Semiconductor
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    Solution and solubility
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    Biosensing techniques
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    Calibration
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    Enzymes, immobilized
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    ION-SELECTIVE ELECTRODES
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    Lipase
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    Miniaturization
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    Models, biological
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    Porosity
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    Semiconductors
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    Triglycerides