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Microwave-Assisted Synthesis of Quasi-Pyramidal CuInS2–ZnS Nanocrystals for Enhanced Near-Infrared Targeted Fluorescent Imaging of Subcutaneous Melanoma
Kavitha Govarthanan,
Published in Wiley-VCH Verlag
2019
Volume: 3
   
Issue: 1
Abstract

Near-infrared (NIR) fluorescent CuInS2–ZnS nanocrystals (CIZS NCs) are synthesized via an ultra-fast, non-injection microwave (MW)-assisted nanoalloying process at 230 ºC within 5 min using 1-dodecanethiol (DDT) as both the sulfur source and solvent under solvothermal (ST) condition. The structural and surface analyses reveal that DDT-functionalized CIZS NCs exhibit quasi-pyramids of tetragonal-phase with well-defined facets. The DDT-functionalized CIZS NCs present a photoluminescence quantum yield (PLQY) of 76% and a long-lived fluorescence lifetime of ≈0.6 µs in organic-phase. Subsequently, DDT-functionalized CIZS NCs are phase-transferred via ligand-exchange using 11-mercaptoundecanoic acid (MUA) into water-soluble MUA–CIZS NCs that exhibit a substantial PLQY of 55%. In addition, the NIR-fluorescent MUA-functionalized CIZS NCs in conjugation with folic acid (FA), as a tumor-targeting ligand, demonstrates enhanced tumor-targeted imaging ability. The FA–MUA–CIZS NC conjugates exhibit a cell viability of ≈75% even at the highest concentration of 1 mg mL–1 and a labeling efficiency of 95.4%. The in vivo imaging results corroborate that FA–MUA–CIZS NCs conjugates are actively targeted to folate receptor-positive B16F10 tumor-bearing C57BL/6 mice in 2 h. The histopathological and hematological studies confirm no significant changes in tissue architecture and blood biochemical parameters. The confocal microscopy studies reveal deep penetration and uniform distribution of FA–MUA–CIZS NCs conjugates in subcutaneous melanoma. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

About the journal
JournalData powered by TypesetAdvanced Biosystems
PublisherData powered by TypesetWiley-VCH Verlag
ISSN23667478
Open AccessYes