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MicroRNA-214 suppresses growth, migration and invasion through a novel target, high mobility group AT-hook 1, in human cervical and colorectal cancer cells
Published in Nature Publishing Group
2016
PMID: 27537384
Volume: 115
   
Issue: 6
Pages: 741 - 751
Abstract
Background: MicroRNA-214 (miR-214) has been shown to act as a tumour suppressor in human cervical and colorectal cancer cells. The aim of this study was to experimentally validate high mobility group AT-hook 1 as a novel target for miR-214-mediated suppression of growth and motility. Methods: HMGA1 and miR-214 expression levels were estimated in cervical and colorectal clinical specimens using qPCR. HMGA1 3′ untranslated region luciferase assays were performed to validate HMGA1 as a target of miR-214. Effect of altering the expression of miR-214 or HMGA1 on proliferation, migration and invasion of human cervical and colorectal cancer cells was investigated. Results: miR-214 expression was poor while that of HMGA1 was high in cervical and colorectal cancer tissues. miR-214-re-expression or HMGA1 downregulation inhibited proliferation, migration and invasion of cancer cells while miR-214 inhibition had opposite effects. miR-214 was demonstrated to bind to the wild-type 3′ untranslated region of HMGA1 but not with its mutant. Conclusions: Low expression of miR-214 concurrent with elevated levels of HMGA1 may contribute to cervical and colorectal cancer progression. miR-214-mediated regulation of HMGA1 is a novel mechanism for its tumour-suppressive actions in human cervical and colorectal cancer cells and opens up avenues for novel therapeutic strategies for these two cancers. © 2016 Cancer Research UK.
About the journal
JournalBritish Journal of Cancer
PublisherNature Publishing Group
ISSN00070920
Open AccessYes
Concepts (63)
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    HIGH MOBILITY GROUP AT HOOK 1
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    HIGH MOBILITY GROUP PROTEIN
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    MICRORNA 214
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    Small interfering rna
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    Unclassified drug
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    3' UNTRANSLATED REGION
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    HIGH MOBILITY GROUP A1A PROTEIN
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    Microrna
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    MIRN214 MICRORNA, HUMAN
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    RNA
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    Tumor protein
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    3' UNTRANSLATED REGION
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    Article
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    Cancer inhibition
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    Cell invasion
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    Cell migration
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    Cell proliferation
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    Colorectal cancer
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    Concentration (parameters)
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    Controlled study
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    Down regulation
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    ECTOPIC EXPRESSION
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    Female
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    Gene expression
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    Human
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    Human cell
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    Human tissue
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    LUCIFERASE ASSAY
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    Polymerase chain reaction
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    Priority journal
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    Protein targeting
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    Uterine cervix cancer
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    Wild type
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    Antagonists and inhibitors
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    Biosynthesis
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    CARCINOMA
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    Cell motion
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    COLORECTAL TUMOR
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    Gene expression regulation
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    Gene silencing
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    Genetics
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    Male
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    Pathology
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    Physiology
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    Rna interference
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    Tumor cell line
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    Tumor invasion
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    Uterine cervix tumor
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    3' UNTRANSLATED REGIONS
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    CARCINOMA
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    Cell line, tumor
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    Cell movement
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    COLORECTAL NEOPLASMS
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    GENE EXPRESSION REGULATION, NEOPLASTIC
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    GENE KNOCKDOWN TECHNIQUES
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    HMGA1A PROTEIN
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    Humans
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    Micrornas
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    Neoplasm invasiveness
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    Neoplasm proteins
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    RNA, NEOPLASM
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    Rna, small interfering
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    Uterine cervical neoplasms