The Special Issue of Micromachines entitled “Micro/Nanofluidic Devices for Single Cell Analysis” covers recent advancements regarding the analysis of single cells by different microfluidic approaches. To understand cell to cell behavior with their organelles and their intracellular biochemical effect, single cell analysis (SCA) can provide much more detailed information from small groups of cells or even single cells, compared to conventional approaches, which only provide ensemble-average information of millions of cells together. Earlier reviews provided single cell analysis using different approaches [1–3]. The author demonstrates invasive and noninvasive with time and non-time resolved SCA [1]; whereas some other literature provided destructive (with dyes, DNA, RNA, proteins and amino acids) and nondestructive (electroporation, impedance measurement and fluorescence based methods) cellular content analysis using microfluidic devices [3]. Further literature also suggest that single cell analysis is possible with capillary electrophoresis (CE) combined with a detection method such as electrochemical detection (ED), laser induced fluorescence (LIF) detection and mass spectrometry (MS) [4,5].