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Metabolic relevance of selenium in the insect Corcyra cephalonica - Uptake of75Se and subcellular distribution
Kalyanaraman Lalitha
Published in Humana Press
1994
PMID: 7946914
Volume: 41
   
Issue: 3
Pages: 217 - 233
Abstract
Requirement, uptake, and subcellular distribution of Na275SeO3 in the larvae of the insect C. cephalonica was investigated. That Se is well tolerated by C. cephalonica upto an added level of 2 ppm in the diet is suggested by the observed increase in body weight, total protein, and succinate dehydrogenase levels. Significant increases in the State 3 respiration ensued with Se supplementation up to 2 ppm in the mitochondrial oxidation of D-glycerol 1-phosphate, succinate and NADH, along with concomitant unaltered State 4 respiration, leading to enhanced RCR values. Maximal uptake of75Se was registered in the larvae maintained on basal diet when subjected to short-term exposure to 0.5 ppm75Se level. When exposure level was further increased up to 20 ppm, the observed decrease in the uptake of75Se suggested that Se status of larvae itself controlled the tissue uptake. Subcellular distribution pattern revealed maximal incorporation of75Se (cpm/g tissue) in the supernatant fraction, whereas, maximal specific75Se activity (cpm/mg protein) was associated with the mitochondrial fraction. Autoradiography of the soluble fractions indicated the presence of single selenoprotein in the larval group with short term 2 ppm75Se exposure. Inherent Se controls both the extent and the nature of distribution of mitochondrial75Se incorporation. Uptake of45Ca by the insect mitochondria was enhanced by dietary Se up to 2 ppm but was unaffected by addition of in vitro75Se in the medium. A more fundamental role for Se in the mitochondrial energy metabolism emerges from these studies. © 1994 Humana Press Inc.
About the journal
JournalBiological Trace Element Research
PublisherHumana Press
ISSN01634984
Open AccessNo
Concepts (24)
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    CALCIUM 45
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    GLYCEROPHOSPHATE
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    Protein
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    Reduced nicotinamide adenine dinucleotide
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    SELENIUM 75
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    SODIUM SELENITE
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    Succinate dehydrogenase
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    Article
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    Cellular distribution
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    DIETARY INTAKE
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    Insect
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    Larva
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    Mitochondrial membrane
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    MITOCHONDRIAL RESPIRATION
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    Nonhuman
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    Weight gain
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    Animal
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    Calcium
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    INSECTS
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    Mitochondria
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    Oxygen consumption
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    Selenium
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    SELENIUM RADIOISOTOPES
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    Tissue distribution