Influence of nitrogen content on low cycle fatigue life and fracture behavior of 316LN stainless steel (SS) alloyed with 0.07 to 0.22 wt pct nitrogen is presented in this paper over a range of total strain amplitudes (±0.25 to 1.0 pct) in the temperature range from 773 K to 873 K (500 °C to 600 °C). The combined effect of nitrogen and strain amplitude on fatigue life is observed to be complex i.e., fatigue life either decreases/increases with increase in nitrogen content or saturates/peaks at 0.14 wt pct N depending on strain amplitude and temperature. Coffin-Manson plots (CMPs) revealed both single-slope and dual-slope strain-life curves depending on the test temperature and nitrogen content. 316LN SS containing 0.07 and 0.22 wt pct N showed nearly single-slope CMP at all test temperatures, while 316LN SS with 0.11 and 0.14 wt pct N exhibited marked dual-slope behavior at 773 K (500 °C) that changes to single-slope behavior at 873 K (600 °C). The changes in slope of CMP are found to be in good correlation with deformation substructural changes. © 2014 The Minerals, Metals & Materials Society and ASM International.