The kinetics of enzymatic hydrolysis of rice bran oil in organic solvent (n-hexane) by free and immobilized Candida lipolytica lipase were investigated in a batch reactor. The n-hexane does not alter pH and temperature optima, but an increase in n-hexane concentration decreases the enzyme activity. The kinetic model proposed to describe the inhibitory effect of solvents on lipolysis was found to fit our data well. © 1994.

P. Padmini

Anantharanganathan Baradarajan

TRIACYLGLYCEROL LIPASE

Vegetable oil

article

Bioreactor

Enzyme kinetics

Hydrolysis

nonhuman

Rice

Yarrowia lipolytica

IIT Madras is a public technical and research university located in Chennai, Tamil Nadu. Founded in 1959, it is recognised as an Institute of National Importance.

IIT Madras has been ranked as the top engineering institute in India for four years in a row by the National Institutional Ranking Framework of the MHRD

It currently offers undergraduate, postgraduate and research degrees across 16 disciplines in Engineering, Sciences, Humanities and Management. About 596 faculty belonging to science and engineering departments and centres of the Institute are engaged in teaching, research and industrial consultancy.

IIT Madras

Kinetics of enzymatic hydrolysis of rice bran oil in organic system

Enzyme and Microbial Technology

A change of the reaction rate was observed for the lipasecatalysed hydrolysis of ricebran oil in a batch stirred tank reactor using immobilized lipase enzyme as compared to free enzyme. The reactor rate was observed to be controlled mainly by factors like temperature, pH, initial enzyme concentration, initial substrate concentration and initial products concentration. © 1993 Springer-Verlag.

Bioprocess Engineering

Lipase catalysed hydrolysis of ricebran oil by free and immobilised enzyme system in batch stirred reactor

The immobilization of lipase from Candida lipolytica on alumina by adsorption for the hydrolysis of ricebran oil is described. Some of the factors which influence the activity of immobilizate and the stability of immobilizate are discussed. About 69% of the initially added enzyme activity is found in the biocatalyst when immobilized at pH 7.2. The stability of the immobilized enzyme to different pH and temperature conditions has also been studied. © 1993 Springer-Verlag.

Studies on immobilization of lipase on alumina for hydrolysis of ricebran oil

Enzyme microb. technol.

Biotechnology and Bioengineering

Rules for optimization of biocatalysis in organic solvents

Journal of the American Oil Chemists’ Society

A simple and rapid colorimetric method for determination of free fatty acids for lipase assay

Journal	Enzyme and Microbial Technology
ISSN	01410229
Open Access	No