Polysaccharide degrading enzymes are hydrolytic enzymes, which have a lot of industrial potential and also play a crucial role in carbon recycling. Pectinases, chitinases and glucanases are the three major polysaccharide degrading enzymes found abundantly in nature and these enzymes are mainly produced by fungal strains. Production of these enzymes by yeasts is advantageous over fungi, because the former are easily amenable to genetic manipulations and time required for growth and production is less than that of the latter. Several yeasts belonging to Saccharomyces, Pichia, Rhodotorula and Cryptococcus produce extracellular pectinases, glucanases and chitinases. This chapter emphasizes on the biological significance of these enzymes, their production and their industrial applications. © 2009 Springer Netherlands.

Sathyanarayana Naidu Gummadi

Department Of Biotechnology

IIT Madras is a public technical and research university located in Chennai, Tamil Nadu. Founded in 1959, it is recognised as an Institute of National Importance.

IIT Madras has been ranked as the top engineering institute in India for four years in a row by the National Institutional Ranking Framework of the MHRD

It currently offers undergraduate, postgraduate and research degrees across 16 disciplines in Engineering, Sciences, Humanities and Management. About 596 faculty belonging to science and engineering departments and centres of the Institute are engaged in teaching, research and industrial consultancy.

IIT Madras

Industrially Important Carbohydrate Degrading Enzymes from Yeasts: Pectinases, Chitinases, and β-1,3-Glucanases

Yeast Biotechnology: Diversity and Applications

Response surface methodology was used to study the effect of microbiological parameters, viz., plate age, inoculum age and amount of inoculum on production of pectin lyase and pectate lyase. Individual optimum values of plate age, inoculum age and amount of inoculum were found to be 31.5 h, 12 h and 5.3% (v/v), respectively for PL and 27 h, 14 h and 4.2% (v/v), respectively for PGL. At optimal conditions of PL 12.7 and 10.5 U mL -1 of PL and PGL was produced and at optimal conditions of PGL 12.2 and 10.4 U mL -1 of PL and PGL was obtained. Since, there is no much difference in PL and PGL production at two different optimal conditions, it can be concluded that enzyme production is not affected in the range of optimal conditions obtained in this study. Hence, the individual optimal values of microbiological parameters for PL or PGL can be used to develop seed culture for maximum production of both PL and PGL. At optimal conditions the production of PL and PGL was increased by 1.2 fold, respectively. © 2010 Academic Journals Inc.

Research Journal of Microbiology

The effect of microbiological parameters on production of pectin lyase and pectate lyase by Debaryomyces nepalensis: A statistical approach

In this study, Debaryomyces nepalensis NCYC 3413 isolated from rotten apple was studied for its halotolerance and its growth was compared with that of Saccharomyces cerevisiae in high salt medium. The specific growth rate of D. nepalensis was not affected by KCl even up to a concentration of 1 M, whereas NaCl and LiCl affected the growth of D. nepalensis. Among all tested salts, LiCl showed maximum inhibition on growth. At all conditions, halotolerance of D. nepalensis was much higher than that of S. cerevisiae. D. nepalensis showed maximum viability (80-100%) when grown in KCl, which was higher than with NaCl and LiCl. Pectinase production by D. nepalensis was noted at all high salt concentrations, namely, 2 M NaCl, 2 M KCl, and 0.5 M LiCl, and the maximum specific activity was observed when the strain was grown in 2 M NaCl. © 2007 Springer Science+Business Media, LLC.

Current Microbiology

Effect of salts on growth and pectinase production by halotolerant yeast, Debaryomyces nepalensis NCYC 3413

Debaryomyces nepalensis was previously isolated, which is capable of producing pectin lyase and pectate lyase using pectin as sole source of carbon. The parameters affecting the activity of these enzymes are categorized into two, viz., chemical (amount of substrate and enzyme) and physical (pH of reaction mixture and temperature of incubation). The effect of these parameters on pectin lyase and pectate lyase was studied using central composite design. The optimal conditions of amount of substrate and enzyme (culture broth) were found to be 545 μl (∼1 g/l pectin) and 123 μl for pectin lyase and 707 μl (∼1.1 g/l polygalacturonic acid) and 96 μl for pectate lyase. The optimum pH and temperature for reaction was found to be 6.4 and 35 °C for pectin lyase and 7.5 and 32 °C for pectate lyase. After optimization, the activity of pectin lyase and pectate lyase was increased by 1.3- and 1.4-fold, respectively. © 2006 Elsevier B.V. All rights reserved.

Biochemical Engineering Journal

Optimization of chemical and physical parameters affecting the activity of pectin lyase and pectate lyase from Debaryomyces nepalensis: A statistical approach

Journal	Data powered by TypesetYeast Biotechnology: Diversity and Applications
Publisher	Data powered by TypesetSpringer Netherlands
Open Access	No