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Improved production of laccase by Daedalea flavida: Consideration of evolutionary process optimization and batch-fed culture
Published in Springer Verlag
2014
PMID: 23903597
Volume: 37
   
Issue: 3
Pages: 493 - 503
Abstract
The genetic algorithm was used effectively to find the optimal values of eight process variables for the maximum laccase production by Daedalea flavida in a stationary culture. The algorithm was modified suitably to improve laccase production with 18 parallel experiments in 4 generations. A high enzyme titer of 65 % was achieved after the optimization and compared to the titer obtained before optimization. To study the effect of the surface immobilized growth on the enzyme production, the fungus was grown on three solid carriers. When cultured on polymer composite fibers, polyurethane foam, or steel wool, at least 2.5 times more biomass was produced, compared to the biomass produced in support-free growth. On the contrary, the mycelia grown on solid support produced much less laccase than non-adhering mycelia. Four parallel runs of batch-fed cultures were done, using the cell mass of D. flavida to evaluate the influence of four different volumes of medium exchanged on laccase production. For sustainable production of the enzyme, complete exchange of medium was favorable, where the laccase activity increased continuously in six consecutive cycles, though, 50 % exchange of medium produced the maximum laccase in terms of mean enzyme activity obtained in six cycles. © 2013 Springer-Verlag.
About the journal
JournalData powered by TypesetBioprocess and Biosystems Engineering
PublisherData powered by TypesetSpringer Verlag
ISSN16157591
Open AccessNo
Concepts (45)
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    Ecology
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    Genetic algorithms
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    Optimization
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    Steel fibers
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    COMPLETE EXCHANGE
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    DAEDALEA FLAVIDA
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    EVOLUTIONARY PROCESS
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    LACCASE PRODUCTION
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    Laccases
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    Polymer composite
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    Process variables
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    Sustainable production
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    Enzymes
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    LACCASE
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    POLYURETHAN FOAM
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    Steel
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    LACCASE
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    Article
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    Biomass production
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    Comparative study
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    Controlled study
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    CULTURE OPTIMIZATION
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    DAEDALEA FLAVIDA
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    Enzyme activity
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    Enzyme immobilization
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    Enzyme synthesis
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    Fed batch culture
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    FUNGAL CELL
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    Fungus
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    Fungus growth
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    Genetic algorithm
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    Mycelium
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    Nonhuman
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    Priority journal
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    Surface property
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    Algorithm
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    Biosynthesis
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    Enzymology
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    Evolution
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    Growth, development and aging
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    POLYPORALES
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    Algorithms
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    Biological evolution
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    LACCASE
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    POLYPORALES