Header menu link for other important links
X
Generation of clinical-grade red blood cells from human umbilical cord blood mononuclear cells
Soma Guhathakurta, Dillip Kumar Bishi,
Published in Springer Verlag
2019
PMID: 30284087
Volume: 375
   
Issue: 2
Pages: 437 - 449
Abstract
A xeno-free method for ex vivo generation of red blood cells (RBCs) is attempted in order to replicate for large-scale production and clinical applications. An efficient milieu was formulated using injectable drugs substituting the animal-derived components in the culture medium. Unfractionated mononuclear cells isolated from human umbilical cord blood were used hypothesizing that the heterogeneous cell population could effectively contribute to erythroid cell generation. The strategy adopted includes a combination of erythropoietin and other injectable drugs under low oxygen levels, which resulted in an increase in the number of mature RBCs produced in vitro. The novelty in this study is the addition of supplements to the medium in a stage-specific manner for the differentiation of unfractionated umbilical cord blood mononuclear cells (MNCs) into erythropoietic lineage. The erythropoietic lineage was well established by day 21, wherein the mean cell count of RBCs was found to be 21.36 ± 0.9 × 10 8 and further confirmed by an upregulated expression of CD235a + specific to RBCs. The rationale was to have a simple method to produce erythroid cells from umbilical cord blood isolates in vitro by mitigating the effects of multiple erythroid-activating agents and batch to batch variability. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
About the journal
JournalData powered by TypesetCell and Tissue Research
PublisherData powered by TypesetSpringer Verlag
ISSN0302766X
Open AccessNo
Concepts (42)
  •  related image
    CD235A ANTIGEN
  •  related image
    ERYTHROPOIETIN
  •  related image
    LYMPHOCYTE ANTIGEN
  •  related image
    Unclassified drug
  •  related image
    Oxygen
  •  related image
    Article
  •  related image
    Cell differentiation
  •  related image
    CELL ISOLATION
  •  related image
    Cell lineage
  •  related image
    Cell proliferation
  •  related image
    Controlled study
  •  related image
    DISEASE TRANSMISSION
  •  related image
    Erythrocyte
  •  related image
    Erythrocyte count
  •  related image
    ERYTHROPOIESIS
  •  related image
    EX VIVO STUDY
  •  related image
    Gene expression regulation
  •  related image
    Human
  •  related image
    Human cell
  •  related image
    In vitro study
  •  related image
    Mononuclear cell
  •  related image
    Priority journal
  •  related image
    Risk factor
  •  related image
    UMBILICAL CORD BLOOD CELL
  •  related image
    UMBILICAL CORD BLOOD MONONUCLEAR CELL
  •  related image
    Cell culture
  •  related image
    CELL CULTURE TECHNIQUE
  •  related image
    Cytology
  •  related image
    Female
  •  related image
    FETUS BLOOD
  •  related image
    IMMUNOPHENOTYPING
  •  related image
    Metabolism
  •  related image
    Preservation
  •  related image
    Procedures
  •  related image
    Cell culture techniques
  •  related image
    Cells, cultured
  •  related image
    Erythrocytes
  •  related image
    FETAL BLOOD
  •  related image
    Humans
  •  related image
    IMMUNOPHENOTYPING
  •  related image
    LEUKOCYTES, MONONUCLEAR
  •  related image
    PRESERVATION, BIOLOGICAL