Muscle fatigue analysis finds significant applications in the areas of biomechanics, sports medicine and clinical studies. Surface electromyography (sEMG) signals have wide application because of its non invasiveness. By nature, signals recorded using surface electrodes from muscles are highly nonstationary and random. The objective of this work is to analyze muscle related fatigue using sEMG signals and polynomial chirplet transform (PCT). sEMG signals are acquired from biceps brachii muscles of twenty volunteers (Mean (sd): age, 23.5 (4.3) years) in isometric contractions. The initial 500 ms is considered as nonfatigue and final 500 ms of the signals are considered as fatigue zone. Then signals are subjected to polynomial chirplet transform to estimate the time-frequency spectrum. Four features, instantaneous mean frequency (IsMNF), instantaneous median frequency (IsMDF), instantaneous spectral entropy (ISpEn) and instantaneous spectral skewness (ISSkw) are extracted for further analysis. Results show that the PCT is able to characterize the nonstationary and multi component nature of sEMG signals. The IsMNF, IsMDF, ISpEn are found to be high in nonfatigue conditions. Further, all the features are very distinct in muscle nonfatigue and fatigue conditions (p<0.001). This technique can be used in analyzing different neuromuscular disorders.